17β-hydroxy-17-(3-hydroxypropyl)androst-4-ene-4-one

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01 Dec 2000 - 23 Jan 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

17β-hydroxy-17-(3-hydroxypropyl)androst-4-ene-4-one

EC Number:

259-701-7

EC Name:

17β-hydroxy-17-(3-hydroxypropyl)androst-4-ene-4-one

Cas Number:

55542-27-3

Molecular formula:

C22H34O3

IUPAC Name:

17 beta-Hydroxy-17 alpha-(3-hydroxypropyl)-4-androsten-3-one

Details on test material:

- Name of test material (as cited in study report): Hydroxypropyltesto
- Analytical purity: 9l.2 %
- Lot/batch No.: 59012052

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

31.5 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

cell number

Any other information on results incl. tables

Table 1: Inhibition of cell growth in relation of test item concentration after 72 h

Concentration of the test item (mg/l)	Reduction of cell concentration after 72 h (%)
5,9	1
8,9	0
13,4	3
20	18
30,1	47

Table 2: Inhibition of growth rate in relation oftest item concentration after 72 h

Concentration of the test item (mg/I)	Reduction of growth rate after 72 h (%)
5,9	0
8,9	0
13,4	2
20	5
30,1	16

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

For the growth rate no effective concentration (ErC) was calculated, since the inhibition of tbis test parameter did not
exceed 16 % in the present study. The calculated EbC50 was slightly above the solubility limit under the test conditions.

Executive summary:

The acute toxicity of Hydroxypropyltesto to algae (Selenastrum capricornutum) was tested in the growth

inhibition test according to the OECD guideline No. 201.

Since the test item is almost insoluble in water the test was carried out with a saturated solution of the test item and four lower level dilutions. For the toxicity test the algae were exposed to a concentration range of 5.9 mg/l to 30.1 mg/l (concentration of saturation) for 72 hours.

The concentration of the test item in saturated solution was checked by DOC analysis (Dissolved Organie Carbon) at test start.

The saturated solution of the test item, diluted by the inoculum only, with a concentration of 30.1 mg/l caused 47 per cent inhibition of cell growth and 16 per cent inhibition of growth rate.

The growth inhibition test resulted in the following toxicity values. For the growth rate no effective concentration (ErC) was calculated, since the inhibition of tbis test parameter did not exceed 16 % in the present study. The calculated EbC50 was slightly above the solubility limit under the test conditions.

Toxicity values after 72 hours:

EbC20 (0-72 h): 20.2 mg/l

EbC50 (0-72 h): 31.5 mg/l

NOEC: 13.4 mg/l