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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Ready biodegradability of Ebanol according to OECD guideline No. 301F.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The ready biodegradability of Ebanol has been determined by the Respirometric Method (modified MITI Test I) according to the OECD Guidelines for Testing of Chemicals, Method No. 301F.

In the conditions of the test, Ebanol undergoes 66% biodegradation after 28 days.The biodegradation rate reached after the 10 -day window (day 9 to 19) is 58%. However, Ebanol is a mixture of eight diastereoisomers. The degradation curve will therefore be the sum of eight growth curves. The 10-day time window criterion was developed on the assumption that a test substance is degraded according to a “standard” growth curve in ready biodegradability tests.The OECD Guidelines for Testing of Chemicals, Section 3.Part 1: Principles and strategies related to the testing of degradation of organic chemicals (adopted 23 March 2006), states that if a substance consists of “constituents with different chain-lengths, degree and/or site of branching or stereo-isomers, even in their most purified commercial forms” and “it is anticipated that a sequential biodegradation of the individual structures is taking place, then the 10-day window should not be applied to interpret the results of the test.” The 10-day time-window should therefore be ignored as a pass / fail criterion for Ebanol. Thus, Ebanol should be regarded as readily biodegradable according to this test based only on the biodegradation percentage of 66% at day 28.