3,3'-thiodi(propionic acid)

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

The Fate of [14C]Thiodipropionates in Rats

Author:

Reynolds, R.C., Astill, B. D., and Fassett, D.W.

Year:

1974

Bibliographic source:

TOXICOLOGY AND APPLIED PHARMACOLOGY 28, 133-141 (1974)

Materials and methods

Objective of study:

toxicokinetics

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 417 (Toxicokinetics)

Version / remarks:

limited documentation

GLP compliance:

no

Remarks:

predates GLP

Test material

Test material information

Constituent 1

Reference substance name:

3,3'-Thiobispropanoic acid

IUPAC Name:

3,3'-Thiobispropanoic acid

Specific details on test material used for the study:

14]Thiodipropionate, specific activity 1.71 mCi/mmol

Radiolabelling:

yes

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 215 - 325g
- Fasting period before study: yes
- Housing: in glass metabolism cages, for collection of urine, feces and exhaled carbon dioxide
- Individual metabolism cages: yes

Administration / exposure

Route of administration:

other: oral: gavage / feed

Vehicle:

other: ethanol: water 1:1

Duration and frequency of treatment / exposure:

single treatment

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

3.1 mg/kg bw/day

Remarks:

(gavage)

Doses / concentrations 2

Dose / conc.:

551 mg/kg bw/day

Remarks:

(gavage)

Doses / concentrations 3

Dose / conc.:

572 mg/kg bw/day

Remarks:

(gavage)

Doses / concentrations 4

Dose / conc.:

650 mg/kg bw/day

Remarks:

(gavage)

Doses / concentrations 5

Dose / conc.:

241 mg/kg bw/day

Remarks:

(feed)

No. of animals per sex per dose / concentration:

1

Control animals:

no

Positive control reference chemical:

no

Results and discussion

Main ADME results

Main ADME results 1

Type:

absorption

Results:

Complete absorption of thiodipropionic acid

Main ADME results 2

Type:

excretion

Results:

as acid labile conjugate of thiodipropionic acid or free thiodipropionic acid, >90% in urine, less than 5% as carbon dioxide

Metabolite characterisation studies

Metabolites identified:

yes

Details on metabolites:

Dithiopropionic acid is recovered as a acid-labile conjugate if given at a dose of 3.1 mg/kg bw. At 650 mg/kg bw, the major fraction is recovered in the non-conjugated form. The acid-labile conjugate is not or only to a small fraction a glucuronidate. Further identification was not performed.

Any other information on results incl. tables

Cumulative elimination in the rat after gavage and feed application

Rat no.	dose [mg/kg bw]	dose [μCi]	gavage/feed	time [days]	Elimination
					urine	carbon dioxide	feces	total
1	3.1	9.3	gavage	4	90.10%	3.10%	0.50%	93.60%
2	650	9	gavage	4	78.10%	8.20%	0.50%	86.80%
3	572	9.3	gavage	4	84.50%	2.80%	0.90%	88.40%
4	551	8.9	gavage	8	88.50%	7.20%	0.20%	95.90%
5	241	8.2	feed	34	87.40%	3.30%	0.10%	90.70%

 

Applicant's summary and conclusion

Conclusions:

No bioaccumulation potential based on study results.