2-Chloro-5-chloromethylpyridine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jan to Feb 1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Version / remarks:

1987

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

454-800-3

EC Name:

-

Cas Number:

70258-18-3

Molecular formula:

C6H5Cl2N

IUPAC Name:

2-chloro-5-(chloromethyl)pyridine

Test material form:

solid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Versuchstierzucht Winkelmann, Borchen, Germany
- Age at study initiation: 9 weeks (males), 14-16 weeks (females)
- Weight at study initiation: 208-237 g (males), 207-228 g (females)
- Housing: 1 animal per cage
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2
- Humidity (%): ca. 50
- Air changes (per hr): ca. 10
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Type of coverage:

occlusive

Vehicle:

physiological saline

Details on dermal exposure:

TEST SITE
- Area of exposure: max. 5 cm x 6 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): treated skin areas were cleaned with soap and water
- Time after start of exposure: ca. 24 hours after start of exposure

Duration of exposure:

24 hours

Doses:

Males: 100, 250, 750, 2000 mg/kg bw
Females: 100, 500 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of weighing: day 1, 4, 8 and 14
- Necropsy of survivors performed: yes

Statistics:

not specified

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

> 250 - < 750 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

> 100 - < 500 mg/kg bw

Based on:

test mat.

Mortality:

Males: After treatment with 100 or 250 mg/kg bw 0/5 or 1/5 animals died within 2 days. After treatment with 750 or 2000 mg/kg bw all animals died (5/5) within 1 day.
Females: After treatment with 100 mg/kg bw no animals died (0/5). After treatment with 500 mg/kg bw all animals died (5/5) within 1 day.

Clinical signs:

other: At 250 mg/kg bw and above male and female animals showed the following clinical signs: Apathy, labored breathing and reddened eyelids. In addition, in males after 2000 mg/kg bw reflex reduction and strong blood circulation of hairless body parts were seen

Gross pathology:

In the animals died after application, the following gross pathological findings were recorded: Liver dark; spleen pale; lungs blown, partly spotted; glandular stomach reddened, partly ulcus-like foci; occasionally stomach filled with liquid mash.

No findings were recorded for the animals killed at the end of the follow-up period.

Other findings:

none specified

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Executive summary:

According to OECD TG 402 the acute dermal toxicity (LD50) in rats is in the range of 250 - 750 mg/kg bw for males and in the range of 100 - 500 mg/kg bw for females. Thus, the test substance is proved to be moderately toxic under the described experimental conditions after acute dermal administration to rats.