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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Guideline:
other: Modified version of the ear/flank technique of Svens (1967)
Principles of method if other than guideline:
Ten female guinea pigs were used (6 test and 4 control).
Induction: Approx 0.1 ml of a 10% (w/v) solution of the test substance in DMF was applied to the outer surface of the ears of the test animals on days 1, 2 and 3. Controls were treated with DMF.
Challenge: on Day 8, immediately prior to the challenge applications, an area of all ten animals was klipped free of hair. Three concentrations of the test substance (10%, 1% and 0.1% w/v solutions in DMF) were used for the challenge. 24 h later the animals were examined and any eythema present at the challenge sites was rated on a four-point scale.
GLP compliance:
not specified
Type of study:
guinea pig maximisation test

Test material

Constituent 1
Chemical structure
Reference substance name:
phenyl N-[2-(phenylsulfanyl)phenyl]carbamate
EC Number:
601-145-8
Cas Number:
111974-73-3
Molecular formula:
C19H15NO2S
IUPAC Name:
phenyl N-[2-(phenylsulfanyl)phenyl]carbamate
Test material form:
solid: crystalline

In vivo test system

Test animals

Species:
guinea pig
Strain:
Dunkin-Hartley
Sex:
female

Study design: in vivo (non-LLNA)

Inductionopen allclose all
Route:
other: dermal application
Vehicle:
other: dimethylformamide
Concentration / amount:
Induction: 10% w/v
Challenge: 10%, 1% and 0.1% w/v)
Challengeopen allclose all
Route:
other: dermal application
Vehicle:
other: dimethylformamide
Concentration / amount:
Induction: 10% w/v
Challenge: 10%, 1% and 0.1% w/v)
No. of animals per dose:
6
Details on study design:
Modified version of the ear/flank technique of Svens (1967). Ten female guinea pigs were used (6 test and 4 control).
Induction: Approx 0.1 ml of a 10% (w/v) solution of the test substance in DMF was applied to the outer surface of the ears of the test animals on days 1, 2 and 3. Controls were treated with DMF.
Challenge: on Day 8, immediately prior to the challenge applications, an area approx 1 cm x 5 cm on each flank of all ten animals was clipped free of hair with a pair of veterinary clippers. Three concentrations of the test substance (10%, 1% and 0.1% w/v solutions in DMF) were used for the challenge. Approx 0.05 ml of each solution was applied, using a glass Pasteur pipette, to separate 1 cm diameter circular areas on both flanks of all ten guinea pigs. 24 h later the animals were examined and any eythema present at the challenge sites was rated on a four-point scale. Any erythema in the pre-treated animals in excess of that in the controls was considered to denote strong skin sensitisation.
Challenge controls:
4
Positive control substance(s):
no

Results and discussion

Any other information on results incl. tables

None of the animals, test or control, showed any erythematous response following challenge with 0.1, 1 or 10% (w/v) solutions of the test substance in DMF.

Applicant's summary and conclusion

Interpretation of results:
not sensitising
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
Not a strong skin sensitiser in the guinea pig.