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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable guideline study, Non-GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Test procedures according to EU Directive 79/831/EEC Annex V, part C: Methods for the determination of the ecotoxicity degradation - biodegradation, Manometric respirometry test. This method is comparable to the OECD guideline 301 F.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Oxooel roh 740

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage. Concentration of dry substance 30 mg/L.
- Laboratory culture: yes
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
63 mg/L
Based on:
DOC
Initial conc.:
90 mg/L
Based on:
test mat.
Details on study design:
TEST CONDITIONS
- pH: 6.6 - 7.4
- Test temperature: room temperature
- Other: nominal concentrations from 45-180 mg/L test substance, 32-127 mg/L DOC


TEST SYSTEM
- Culturing apparatus: glass bottles, closed system
- Number of culture flasks/concentration: 2-3
- Method used to create aerobic conditions: on a magnetic stirrer, aerated with CO2-free air

SAMPLING
- Sampling frequency: daily

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
100
Sampling time:
28 d
Details on results:
- Mean lag time: 3 days
- Time required for 10% degradation: 4 days
- Total degradation at the end of the test: 100 %

Kinetic of test substance (in %):
10.6 after 4 days
57.3 after 9 days
84.6 after 14 days
94.5 after 18 days
> 100 after 23 days

Degradation products: not measured

BOD5 / COD results

Results with reference substance:
Kinetic of control substance (in %):
27.5 after 7 days
69.2 after 21 days

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable