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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study in compliance with GLP-criteria.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Ephedrine hydrochloride
EC Number:
200-074-6
EC Name:
Ephedrine hydrochloride
Cas Number:
50-98-6
IUPAC Name:
2-(methylamino)-1-phenylpropan-1-ol hydrochloride
Test material form:
solid: crystalline
Details on test material:
- Name of test substance:-Ephedrin-Hydrochlorid
- Chemical name: (1R, 2S)-2-Methylamino-l-phenyl-l-propanolhydrochlorid
- Aggregate state: solid, powder
- Colour: white
- Homogeneity: homogeneous
- Special conditions for storage: storage at room temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Activated sludge from laboratory wastewater plants treating municipal sewage.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
34 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS:
- Test temperature: 22 ± 2 °C

PRELIMINARY INVESTIGATIONS:
- TOC (mg/g): 606
- DOC (mg/g): 611
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
- Degradation degree of the reference substance after 14 days (% DOC): 90-100
- Degradation degree in the inhibition control after 14 days (% DOC): 90-100
- Physico-chemical (abiotic) elimination of the test substance (% DOC): <10 at the end of the test
- Elimination of the test substance by adsorption (% DOC): <10 after 5 days
% Degradation
Value:
90 - 100
Sampling time:
18 d
Details on results:
- Duration of the adaptation phase (days): 6
- Duration of the degradation phase (days): 11
- Degradation of the test substance at the end of the 10-day window (% DOC): 90-100
- Degradation degree of the test substance at the end of the test (% DOC): 90-100
- 10-day window criterion met: Yes

Any other information on results incl. tables

DOC decrease (%)

Day

RS

IH

PC

AC

TS1

TS2

TSmean

0

0

0

0

0

0

0

0

1

-4

0

7

3

0

0

0

3

2

3

4

4

1

-1

0

5

93

95

5

0

-3

-1

-2

7

89

95

13

93

4

49

10

88

94

4

95

92

94

14

92

96

3

96

95

96

17

95

98

4

98

98

98

 18  94 97     98  98  98

RS: reference substance

IH: inhibition control

PC: abiotic control

AC: adsorption control

TS: test substance

Applicant's summary and conclusion