Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test item showed a degradation rate of > 60 % within 28 days in the manometric Respirometry Test (GLP, OECD 301F)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The ready biodegradability of the test item was evaluated in the Manometric Respirometry Test (OECD 301 F) on the basis of the BOD (Biological Oxygen Demand) parameter. The degradation rate was calculated as a percentage of the BOD/COD ratio. The COD (Chemical Oxygen Demand) was determined in a variant of the ISO method 6060 (Kelkenberg method). The non adapted inoculum (100 mL effluent/L) was taken from a municipal waste water treatment plant. The test substance concentration was 100 mg COD/L.
In parallel to the standard 28 d test samples (test 1), a second set of samples was run for 91 days (until the plateau was reached) to determine the ultimate degree of biodégradation of the test item (test 2). Under the experimental conditions of the OECD 301 F test, test 1 showed a degradation result of 64 % BOD28/COD. Thus, the test item turned out to be readily biodegradable (degradation level > 60 %). The ultimate oxygen uptake degree was determined to be 84 % BOD/COD after 91 days (test 2)(Henkel, 1999).
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