Methoxyammonium chloride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Reference 2

Reference Type:

other: recalculation with ToxRatPro Version 2.09 (2005)

Title:

Unnamed

Year:

2007

Report date:

2007

Reference 3

Reference Type:

secondary source

Title:

European Union Risk Assessment Report BIS(HYDROXYLAMMONIUM)SULPHATE CAS No: 10039-54-0 EINECS No: 233-118-8,

Author:

ECB

Year:

2008

Bibliographic source:

Final approved version, May 2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 Part 9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Bis(hydroxylammonium) sulphate

EC Number:

233-118-8

EC Name:

Bis(hydroxylammonium) sulphate

Cas Number:

10039-54-0

Molecular formula:

H3NO.1/2H2O4S

IUPAC Name:

bis(hydroxyammonium) sulfate

Details on test material:

- Analytical purity: > 99%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The stock solution (1g/l) was diluted to 10 mg/l. From this stock solution target concentrations were prepared with dilution with test water.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Desmodesmus subspicatus (former name: Scenedesmus subspicatus)
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): Supplier: Sammlung für Algenkulturen der Universität Göttingen
- Age of inoculum (at test initiation): The test algae are taken from a 3 days-old continously growing preculture
- Method of cultivation: The test strain is kept in liquid culture in the laboratory.

ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as test medium

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20°C

pH:

8.1-10

Nominal and measured concentrations:

0, 0.039, 0.078, 0.156, 0.312, 0.625, 1.25, 2.5 and 5 mg/l nominal

Details on test conditions:

TEST SYSTEM
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 20 ml glass tubes with flat bottom, plugged with siliconesponge caps, 10 ml test volume
- Aeration: no
- Initial cells density: 10000 cells/ml
- Control end cells density: yes
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

GROWTH MEDIUM
- Standard medium used: yes


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 201

OTHER TEST CONDITIONS
- Adjustment of pH: not during test
- Photoperiod: continous

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: in vivo chlorophyll-a-fluorescence at 685 nm wavelength after 0, 24, 48, 72 and 96 hours
- pH values: after 0 and 96 hours


TEST CONCENTRATIONS
0.039, 0.078, 0.156, 0.312, 0.625, 1.25, 2.5 and 5 mg/l (nominal). A control was tested in parallel.

Reference substance (positive control):

yes

Remarks:

Potassium chromate p.a.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.156 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Re-calculation of critical concentrations of BASF AG Algae test 289/89 (BASF AG 2007, 02/w289/88)

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

1.043 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Re-calculation of critical concentrations of BASF AG Algae test 289/89 (BASF AG 2007, 02/w289/88)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.529 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Re-calculation of critical concentrations of BASF AG Algae test 289/89 (BASF AG 2007, 02/w289/88)

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.674 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

yield

Remarks on result:

other: Re-calculation of critical concentrations of BASF AG Algae test 289/89 (BASF AG 2007, 02/w289/88)

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.353 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

yield

Remarks on result:

other: Re-calculation of critical concentrations of BASF AG Algae test 289/89 (BASF AG 2007, 02/w289/88)

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.72 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Remarks on result:

other: result of BASF AG 1988

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

0.42 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Remarks on result:

other: result of BASF AG 1988

Any other information on results incl. tables

Summary of results (BASF AG 1988):

96h: EC20 = 0.52; EC50 = 0.86; EC90 = 1.64 mg/l
72h: EC20 = 0.42; EC50 = 0.72; EC90 = 1.24 mg/l
48h: EC20 = 0.38; EC50 = 0.61; EC90 = 3.04 mg/l
24h: EC20 = 0.43; EC50 = 0.92; EC90 = ---- mg/l

No chemical dose verification was performed. All values refer to nominal  test concentrations of Bis(hydroxylammonium) sulphate.

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In a new mathematical evaluation, critical concentrations of BASF AG  Algae test 289/89, has been re-calculated, in order to allow an  assessment based on growth rate and biomass (according to the  requirements given in OECD guideline 201). (BASF AG 2007, 02/w289/88)  

The mathematical evaluation was based on a linear relationship between  fluorescence and cell number and raw data were equated with cell numbers.


Summary of Results for all Endpoints: Critical effect and threshold  concentration as observed at end of experimental time; EC: Effective  concentration for xx% reduction; LOEC: Lowest observed effect  concentration; NOEC: No observed effect concentration

a) Yield
Critical conc (mg/L)/ 0 -24h/0 -48h /0 -72h/0-96h
EC10/ 0.182/ 0.292/ 0.353/ 0.460
EC20/ 0.357/ 0.378 / 0.441/ 0.554
EC50/ 1.279/ 0.619/ 0.674/ 0.792
LOEC/ 0.312/ 0.312/ <0.039/ 0.625
NOEC/ 0.156/ 0.156/ <0.039/ 0.312

b) Growth rate 
EC10/ 0.311/ 0.424/ 0.529/ 0.758
EC20/ 0.412/ 0.526/ 0.668/ 0.917
EC50/ 0.701/ 0.794/ 1.043/ 1.318
LOEC/ 0.312/ 0.625/ 0.312/ 0.625
NOEC/0.156/ 0.312/ 0.156/ 0.312

c) Sectional growth rate 
EC10/ 0.311/ 0.538/ 0.974/ 1.531
EC20/ 0.412/ 0.633/ 1.153/ 1.609
EC50/ 0.701/ 0.865/ 1.589/ 1.768
LOEC/ 0.312/ 0.625/ 2.500/ 2.500
NOEC/ 0.156/ 0.312/ 1.250/ 1.250

d) Biomass integral 
EC10/ 0.284/ 0.300/ 0.330/ 0.393
EC20/ 0.365/ 0.376/ 0.411/ 0.481
EC50/ 0.586/ 0.576/ 0.624/ 0.706
LOEC/ 0.312/ 0.312/ 0.312/ 0.312
NOEC/ 0.156/ 0.156/ 0.156/ 0.156

* for 95% Confidence limits, please see BASF AG (2007), 02/w289/88