Dimethyl phosphonate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented study report which meets basic scientific principles. Adopted according to OECD SIDS (public available peer reviewed source). The original source is available and has been reviewed.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Reference 2

Reference Type:

secondary source

Title:

Unnamed

Year:

2004

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Dimethyl phosphonate was applied undiluted to the ears of rabbits for 1-4 hours.

GLP compliance:

no

Remarks:

GLP was not mandatory at the time of the study.

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl phosphonate

EC Number:

212-783-8

EC Name:

Dimethyl phosphonate

Cas Number:

868-85-9

Molecular formula:

C2H7O3P

IUPAC Name:

dimethyl phosphonate

Details on test material:

- Name of test material (as cited in study report): dimethyl phosphonate
- Physical state: liquid
- Analytical purity: not reported

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 3-4 kg
- Housing: the animals were individually caged.

Test system

Type of coverage:

other: plastic tape

Preparation of test site:

not specified

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

500 µL

Duration of treatment / exposure:

1-4 hour(s)

Observation period:

7 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: ear
- Type of wrap if used: plaster tape

REMOVAL OF TEST SUBSTANCE
- Washing (if done): the test substance was washed away with water and soap/vegetable oil
- Time after start of exposure: after the exposure

SCORING SYSTEM: Draize

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Remarks:

(4-hour exposure)

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.3

Max. score:

4

Reversibility:

other: not applicable

Results 2

Irritation parameter:

erythema score

Remarks:

(2-hour exposure)

Basis:

animal #2

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 7 days

Results 3

Irritation parameter:

erythema score

Remarks:

(1-hour exposure)

Basis:

animal #3

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

fully reversible within: 5 days

Results 4

Irritation parameter:

edema score

Remarks:

(4-hour exposure)

Basis:

animal #1

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Results 5

Irritation parameter:

edema score

Remarks:

(2-hour exposure)

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Results 6

Irritation parameter:

edema score

Remarks:

(1-hour score)

Basis:

animal #3

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: not applicable

Irritant / corrosive response data:

No further data.

Other effects:

No further data.

Any other information on results incl. tables

Table 1. Individual skin examination scores after 1 -4 hour exposure to dimethyl phosphhonate (scoring according to Draize scheme)

Skin effect	Erythema				Edema
Scoring^a	24h	48h	72h	Mean^b	24h	48h	72h	Mean^b
Animal No.10  (4 -hour exposure)	1	0	0*	0.3	0	0	0*	0
Animal No.35 (2 -hour exposure)	2	2	2	2	0	0	0	0
Animal No.77 (1 -hour exposure)	1	1	1	1	0	0	0	0

^aTimepoint of reading after substance removal subsequent to 1-4 hour dermal exposure

^bMean of 24, 48, and 72 hours in intact skin

Individual data were reported for only one animal per time point.

* Reading at 72 hours was not observed. The same scores assumed as for 48h time point (worst case).

On the basis of the results of the present study it can be concluded that dimethyl phosphonate is not a skin irritant, according to DSD and CLP criteria.

Applicant's summary and conclusion

Interpretation of results:

other: not irritating.

Executive summary:

In a primary dermal irritation study, the skin irritation/corrosion potential of dimethyl phosphonate was tested. 500 µL of dimethyl phosphonate was applied undiluted to the ears of 2 New Zealand White rabbits for a period of time of 1 to 4 hours. The rabbits were observed for 7 days. Skin reactions were assessed using the Draize scheme approx. 24, 48, 72 hours after removal of the test substance. The exposure period of 4 hours caused only very slight erythema at 24 hours observation time. No further skin reactions were observed. On the basis of the results of the present study can be concluded that dimethyl phosphonate is not a skin irritant.