Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 213-999-5 | CAS number: 1071-93-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The EC50 for growth rate of Pseudokirchneriella subcapitata at 0–72 h was 8.7–9.19 mg/L. The NOEC for growth rate was 0.22–1.97 mg/L and the EC10 0.17–3.11 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 8.7 mg/L
- EC10 or NOEC for freshwater algae:
- 0.22 mg/L
Additional information
The batch of Adipic acid dihydrazide tested was a white powder with a purity of 99% and the substance was completely soluble in test medium at the concentrations tested.
A final test was performed based on the results of a combined limit/range-finding test. Six exponentially growing algal cultures were exposed to an untreated control, whereas three replicates per group were exposed to 0.32, 1.0, 3.2, 10, 32 and 100 mg Adipic acid dihydrazide per litre. Initial cell density was 104 cells/ml. The total exposure period was 72 hours and samples for analytical
confirmation of exposure concentrations were taken at the start, after 24 and 72 hours of exposure.
The actual concentrations were at the level of nominal (91-98%) at the start of the test. Measured concentrations decreased to 26-73% of initial at the end of the test. The Time Weight Average (TWA) concentrations were calculated to correspond to 0.22, 0.62, 1.7, 5.5, 21 and 81 mg/l at nominally 0.32, 1.0, 3.2, 10, 32 and 100 mg/l, respectively.
The EC50 for growth rate of Pseudokirchneriella subcapitata at 0-72 h was 8.7 - 9.19 mg/L. The NOEC for growth rate was 0.22 - 1.97 mg/L and the EC10 0.17- 3.11 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
