2,2''-dihydroxy-4,4''-(2-hydroxy-propane-1,3-diyldioxy)dibenzophenone

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

OECD 203

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

424-210-0

EC Name:

-

Cas Number:

23911-85-5

Molecular formula:

C29 H24 O7

IUPAC Name:

2-benzoyl-5-[3-(4-benzoyl-3-hydroxyphenoxy)-2-hydroxypropoxy]phenol

Test solutions

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: Dimethylformamide in dechlorinated tap water.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness was approximately 100 mg/l as CaCO3

Test temperature:

The water temperature was controlled at 14°C

Dissolved oxygen:

greater than or equal to 9.2 mg O2/l

Nominal and measured concentrations:

The test concentrations to be used in the definitive study were determined by preliminary range-finding studies.
An initial range finding study was performed whereby fish were exposed to a series of concentrations of 0.020 and 0.20 mg/l under static test conditions. A second range finding study was performed to concentration 0.10 mg/l.
There were no sub-lethal effects of exposure during the range-finding study. The results of the range-finding studies showed no mortalities at the test concentrations of 0.020, 0.1 and 0.20 mg/l. However, precipitation of the test material was observed at 0.20 mg/l indicating 0.10 mg/l to be the maximum limit of water solubility of the test material under these test conditions.
Based on this information, a single test concentration, in duplicate, of 0.10 mg/l was selected for the definitive study. This experimental design conforms to a "Limit test', to confirm that at the highest attainable test concentration of 0.10 mg/l, no mortalities or sub-lethal effects of exposure were observed.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 0.1 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 0.1 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 0.1 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 4

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.1 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 0.1 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.1 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

There were no sub-lethal effects observed in 20 fish exposed to a test concentration of 0.10 mg/l for a period of 96 hours.

Applicant's summary and conclusion

Conclusions:

The acute toxicity of Fadex He 1819 PK to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-Hour LC50 of greater than 0.10 mg/|. Correspondingly the No Observed Effect Concentration was greater than or equal to 0.10 mg/l.