2-nitroaniline

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian cell study: DNA damage and/or repair

Remarks:

Type of genotoxicity: DNA damage and/or repair

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles (no analytical purity, no details given)

Data source

Reference

Reference Type:

publication

Title:

Evaluation of Damage to DNA After in Vivo Exposure to Different Classes of Chemicals.

Author:

Cesarone CF et al.

Year:

1982

Bibliographic source:

Arch. Toxicol., Suppl. 5: 355-359

Materials and methods

Principles of method if other than guideline:

Male Swiss CD1 mice were treated in vivo with a dose of 100 mg/kg bw . Four hours after administration, nuclei were isolated from target tissues (liver and kidney) and lysed on a membrane filter. DNA was eluted using an alkaline buffer as a function of the molecular weight followed by a microfluorometric assay of DNA. Details of this method were described by Cesarone et al. (1979).

Cesarone et al. (1979). Anal. Biochem. 100: 188-197

GLP compliance:

no

Type of assay:

other: Alkaline Elution Technique coupled with a microfluorometric assay for DNA damage

Test material

Test material information

Constituent 1

Reference substance name:

2-nitroaniline

EC Number:

201-855-4

EC Name:

2-nitroaniline

Cas Number:

88-74-4

Molecular formula:

C6H6N2O2

IUPAC Name:

2-nitroaniline

Details on test material:

- Name of test material (as cited in study report): 2-Nitroaniline
- Analytical purity: no data

Test animals

Species:

mouse

Strain:

other: Swiss CD1

Sex:

male

Details on test animals or test system and environmental conditions:

no further data

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

no data

Duration of treatment / exposure:

once

Frequency of treatment:

once

Post exposure period:

4 hrs

Doses / concentrations

Remarks:

Doses / Concentrations:
100 mg/kg bw
Basis:
other: actually administered

No. of animals per sex per dose:

no data

Control animals:

yes, concurrent vehicle

Positive control(s):

no data

Examinations

Tissues and cell types examined:

liver and kidneys

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

valid

Negative controls validity:

not applicable

Positive controls validity:

not applicable

Any other information on results incl. tables

The elution rate (K), expressed in mL-1, is the mean of eight experiments (24 for controls) ± SD and has been computed using the formula:

K = ln (DNA% retained on the filter) / eluted volume

Results:

Compound	Dose	K (E-03) ± SD
	[mg/kg bw]	Liver	Kidney
solvent control	-	21 ± 9	22 ± 10
2-nitroaniline	100	36 ± 4	36 ± 5