Phosphorus

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well performed guideline conform non GLP study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Principles of method if other than guideline:

only one group of animals has been used
a dose of 15000 mg/kg body weight has been used instead of 2000 mg/kg body weight
only female rodents were used because in prior studies sex-related differences had not been noticed

GLP compliance:

no

Remarks:

study performed before GLP guidelines

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

phosphorus

EC Number:

918-594-3

Cas Number:

7723-14-0

Molecular formula:

(P)n (Phosphorus red modification)

IUPAC Name:

phosphorus

Constituent 2

Reference substance name:

Phosphorus

EC Number:

231-768-7

EC Name:

Phosphorus

Cas Number:

7723-14-0

IUPAC Name:

phosphine

Details on test material:

- Name of test material (as cited in study report): Phosphor rot
- Substance type: element
- Physical state: powder
- Stability under test conditions: stable

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: report 131/75
- Weight at study initiation: see table below
- Fasting period before study: 16 hours
- Housing: in plastic cages on wood shavings
- Diet (e.g. ad libitum): Altromin 1324 (Altrogge)
- Water (e.g. ad libitum): ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: 1% starch mucilage

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 15000 mg/kg body weight

Doses:

one dose with 15000 mg/kg body weight

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days (or other?) 14 days
- Frequency of observations and weighing: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: clinical signs, body weight, necropsy

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 15 000 mg/kg bw

Mortality:

no mortality occured during the study

Clinical signs:

other: no clinical signs have been observed

Gross pathology:

No effects

Any other information on results incl. tables

body weight of the rats

Animal no.	sex	dose [mg/kg]	initally body weight	body weight after 7 days	body weight after 14 days
1	female	15000	98	138	160
2	female	15000	90	120	136
3	female	15000	102	144	166
4	female	15000	94	128	150
5	female	15000	94	126	150
6	female	15000	92	126	144
7	female	15000	104	146	172
8	female	15000	96	124	142
9	female	15000	96	138	158
10	female	15000	100	128	144

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 (acute oral) of red phoshorus in female rats is > 15000 mg/kg bw.

Executive summary:

After the administration of the highest applicable amount of 15,000 mg red phosphorus

/kg bw, the all animals survived and showed normal behavior during the 14 days observation time.

The trend in body weight of the animals during the observation period is given in the table above. The necropsy of the killed animals at the end of the observation period did not reveal any macroscopically visible changes.

Based on the current results the specific acute oral toxicity could not be determined. The acute oral LD50 for female rats is for sure above 15000 mg/kg body weight.