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EC number: 213-912-0 | CAS number: 1066-35-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The result was obtained by the valid application of a well-established method. It was not conducted under GLP. Read-across to the registered substance is considered scientifically justified.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
- Reference Type:
- secondary source
- Title:
- Genotoxicity studies on selected organosilicon compounds: In Vitro assays
- Author:
- Isquith A, Matheson D, Slesinski R
- Year:
- 1 988
- Bibliographic source:
- Fd Chem Toxic. Vol 26, No. 3, pp. 255 - 261
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- yes
- Remarks:
- no duplicates though test repeated. Concurrent positive controls did not stain
- Principles of method if other than guideline:
- The procedure used is a modification of that reported by Clive and Spector (Mutation Research, 31:17-29, 1975).
- GLP compliance:
- no
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Dichloro(dimethyl)silane
- EC Number:
- 200-901-0
- EC Name:
- Dichloro(dimethyl)silane
- Cas Number:
- 75-78-5
- Molecular formula:
- C2H6Cl2Si
- IUPAC Name:
- dichloro(dimethyl)silane
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: mouse lymphoma (L5178Y)
- Metabolic activation:
- with and without
- Metabolic activation system:
- Mouse Liver S9
- Test concentrations with justification for top dose:
- without activation was 0.02 - 0.32 ul/ml; with activation the dose range used was 0.04 - 0.64 ul/ml
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Ethanol
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Remarks:
- (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- N-dimethylnitrosamine
- Remarks:
- (with activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- (with activation)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar
DURATION
- Exposure duration: 4 hours
- Expression time (cells in growth medium): 3 days
- Selection time (if incubation with a selection agent): 10 days
NUMBER OF REPLICATIONS: 3 plates per concentration
DETERMINATION OF CYTOTOXICITY
- Method: loss in growth potential of cells (relative total growth) - Evaluation criteria:
- The test substance was evaluated for it's ability to induce point mutations.
Toxicity is defined by a 50 % or greater reduction in growth compared with the solvent control. - Statistics:
- Cells counted. Relative suspension growth (% of control), total mutant and viable clones, relative cloning efficiency, % relative growth and mutant frequency calculated.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: 0.64 ul/ml with activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- COMPARISON WITH HISTORICAL CONTROL DATA: Positive controls were within range of historical control data
- Remarks on result:
- other: strain/cell type: mouse lymphoma assay (L5178Y)
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 2 : Results of Mammalian Mutagenicity assay with L5178Y Mouse Lymphoma cells
Concentration µg/ml |
Mutant* Frequency |
Mutant* Frequency |
%Relative Growth. |
%Relative Growth. |
Cytotoxicity |
|
— MA |
+ MA |
— MA |
+ MA |
- |
Solvent Control |
13.3 |
38.8 |
100 |
100 |
No |
Negative Control |
21.7 |
12.5 |
113.2 |
113.8 |
No |
0.02 |
16.2 |
- |
114.8 |
- |
No |
0.04 |
29.6 |
24.6 |
55 |
87.5 |
No |
0.08 |
26.1 |
27.2 |
102.7 |
99.1 |
No |
0.16 |
14.2 |
33.7 |
67 |
91 |
No |
0.32 |
23.1 |
26.3 |
79.4 |
103.8 |
No |
0.64 |
- |
21.7 |
- |
23.9 |
Yes |
Positive Control |
508.5 |
221.5 |
25.7 |
28.2 |
Yes |
*Per 104 surviving cells
Solvent control with Ethanol
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
Dimethyldicholrosilane did not induce point mutations at any dose level, with or without metabolic activation. The test substance is non-mutagenic in mouse lymphoma (L5178Y) cells under the conditions of the test , .
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