Barium titanium trioxide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1962

Reliability:

2 (reliable with restrictions)

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Acute Oral and Parenteral Toxicity of four Titanate Compounds in the Rat

Author:

J.R. Brown, E. Mastromatteo

Year:

1962

Bibliographic source:

Industrial Medicine and Surgery, July 1962 302-304, Number 7, Volume XXXI

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Known dry weights of titanates were mixed with corn oil to form a 40% suspension. This was diluted for use as required in order that each animal should receive the same total volume in proportion to its body weight.

GLP compliance:

no

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

Barium titanium trioxide

EC Number:

234-975-0

EC Name:

Barium titanium trioxide

Cas Number:

12047-27-7

Molecular formula:

Ba.O3Ti

IUPAC Name:

titanium(4+) barium(2+) trioxidandiide

Details on test material:

Typical analysis (app. percent)
BaO: 63,7
TiO2: 33,21
SiO2: 0,35
Ign. Loss: 1,33

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

Young adult male Wistar rats weighing 200 grams or more were used. They were obtained from a commercial breeder. The animals were housed in seperate wire cages and supplied with water and a standard fox cube diet at libitium.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: corn oil

Details on oral exposure:

Because of the insolubiltiy of the titanates, corn oil was used as a vehicle for adminstration. Known dry weights of titanates were mixed with corn oil to form a 40% suspension. This was diluted for use as required in order that each animal should receive the same total volume in proportion to its body weight. For the determination of acute oral toxicity the sample was adminstered directly into the stomach in calculated amounts using a rubber catheder stomach tube and a graduated syringe.

Doses:

Dose levels:
1.5 g/kg bw
3.0 g/kg bw
6.0 g/kg bw
12.0 g/kg bw

No. of animals per sex per dose:

6 male animals per dose

Control animals:

yes

Details on study design:

All animales were maintend and observed for one month following observation of test materials. The time of death and any evidence of toxic effect were noted. The same amount of corn oil alone were given to a similar number of rats in each case of control.
Rats that died as a result of the adminstation of titantate were examined for evidence of toxic effects. Gross findings doses were first carried out by both oral and intraperitonal LD50 doses with their 95% fiducial limits, groups of six animal were given four dose level which varied by a constant geometric factor. those dose levels were based on the range finding data. The LD50 dose was calculated according to the tables and instructions given by Weil

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD0

Effect level:

1 500 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LD0

Effect level:

3 000 mg/kg bw

Based on:

test mat.

Effect levels 3

Sex:

male

Dose descriptor:

LD0

Effect level:

6 000 mg/kg bw

Based on:

test mat.

Effect levels 4

Sex:

male

Dose descriptor:

LD0

Effect level:

12 000 mg/kg bw

Based on:

test mat.

Mortality:

All animales were maintend and observed for one month following observation of test materials. The time of death and any evidence of toxic effect were noted. Rats that died as a result of the adminstation of titantate were examined for evidence of toxic effects.
None of the rats died as a result of oral adminstration despite quite high dosage rates.

Clinical signs:

other: At the higher dosage levels with both routes of administration the rats were lethargic for the first few hours. Other findings on the high dosage levels were: reduced activity, temporary loss of appetite, and brownish colored discharge from the nose and e

Gross pathology:

Histologic study showed no specific changes .
There was evidence of inflammatory change in the liver, kidney, spleen, and lungs. There were also hemorrhagic areas noted in the kidneys

Any other information on results incl. tables

Table II	Acute Oral Toxicity Ratio of Rats killed those treated at graduated dose levels (grams of titanate compound per kilogram of body weigth)
Compound	Dose Level (gms/kg)
	1,5	3,0	6,0	12,0
Barium Titanate	0/6	0/6	0/6	0/6

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

LD50 >5000 mg/kg bw. Hence, barium titanate need not be classified according to GHS.

Executive summary:

The minimal lethal doses by the oral route exceeds 12g/kg in the rat.