2,3-dichlorobuta-1,3-diene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-04-16 till 2010-05-31

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline and GLP-compliant study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

The size of some of the fish used in the study exceeded the size specified in the guideline. However, this devation is considered to have had no effect on the outcome of the study. In addition the temperature was slightly outside the acceptable range of

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,3-dichloro-1,3-butadiene

IUPAC Name:

2,3-dichloro-1,3-butadiene

Constituent 2

Reference substance name:

2,3-dichlorobuta-1,3-diene

EC Number:

216-721-0

EC Name:

2,3-dichlorobuta-1,3-diene

Cas Number:

1653-19-6

Molecular formula:

C4H4Cl2

IUPAC Name:

2,3-dichlorobuta-1,3-diene

Constituent 3

Reference substance name:

2,3-dichlorobutadiene-1,3

IUPAC Name:

2,3-dichlorobutadiene-1,3

Details on test material:

Partie number - Q101
Sample number - 1152
Purity - not stated

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken from freshly prepared control and test media samples at 0, 24, 48 and 72 hours, samples of the corresponding 24 hour aged media were taken after 24, 48, 72 and 96 hours.

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution was prepared daily, 847 uL (or 424 uL) of the test substance was added to 10 L (or 5 L) of dilution water and mixed for two minutes with a magnetic stirrer. Following stirring any undissolved particles were removed by filtration through a 7 - 12 um pore size filter paper. The pH of the stock solution was 7.8. The test media were prepared by addition of appropriate volumes of the stock solution to test vessels and dilution to 5000 mL with water. A single replicate was used for each test treatment and contained seven fish.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The fish were obtained from Interaquaristic.de on 23 October 2009 and acclimated to the test conditions until used for the study. Fish were fed daily until 24 hours prior to the start of the study. The standard length of the fish was 3.41 cm with a standard deviation of 0.16 cm, n = 35

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Test conditions

Hardness:

Dilution water - 250 mg/L as CaCO3

Test temperature:

Temperature range during the study was 20.6 - 21.7 °C. The temperature measured in the control vessel at the end of the end of the exposure period was 19.4°C, this was the only time the temperature fell below 20°C. This minor deviation is not condiered to have had nay effect on the outcome of the study.

pH:

pH range during the study was 7.5 - 7.8

Dissolved oxygen:

Dissolved oxygen (as % air saturation value) range during the study was 75 - 99%

Salinity:

Not applicable

Nominal and measured concentrations:

0 (control), 9.4, 20.7, 45.5 and 100 mg/L nominal concentrations. Geometric mean concentrations were determined for each 24 hour exposure period for each test concentration, the results are presented in the results section.

Details on test conditions:

A semi-static study was performed with renewal of the test substance every 24 hours.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.39 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.501 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Any other information on results incl. tables

Sublethal observations / clinical signs:

Measured concentrations of test material

Time (h)	Sample	Measured concentration (mg/L)
		9.4	20.7	45.5	100
0	new	0.71	1.365	3.396	10.216
24	old	0.315 *	0.637	1.004	5.507
	new	0.813	2.368	4.949	ns
48	old	0.315 *	0.974	3.100	ns
	new	1.094	2.474	ns	ns
72	old	0.315 *	1.086	ns	ns
	new	0.646	1.344	ns	ns
96	old	0.315 *	0.699	ns	ns

* Half of limit of quantification
ns – not sampled as all fish dead

 

Calculated geometric means

Time (h)	Measured concentration (mg/L)
	9.4	20.7	45.5	100
0	0.710	1.365	3.396	10.216
24	0.473	0.932	1.847	7.501
48	0.489	1.190	2.689	7.501
72	0.520	1.324	2.689	7.501
96	0.502	1.225	2.689	7.501

 

 

Cumulative mortality (%)

Time (h)	Nominal concentration (mg/L)
	Control	9.4	20.7	45.5	100
2	0	0	0	0	0
24	0	0	14	57	100
48	0	0	14	100	100
72	0	0	14	100	100
96	0	0	14	100	100

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 hr LC50 based on the geometric mean measured concentrations was 1.39 mg/L, the corresponding no-observed effect concentration (NOEC) was observed to be 0.501 mg/L.

Executive summary:

Zebra fish were exposed to nominal 2,3-dichlorobutadiene-1,3 concentrations of 0 (control), 9.4, 20.7, 45.5 and 100 mg/L for 96 hours under semi-static conditions according to EU Method C.1 Acute toxicity to fish (2008). Analysis of new and 24 hour aged media was performed for each treatment and geometric mean values were calculated for each 24 hour exposure period. The 96 hr LC₅₀based on the geometric mean measured concentrations was 1.39 mg/L, the corresponding no-observed effect concentration (NOEC) was observed to be 0.501 mg/L.