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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Amines, coco alkyl
EC Number:
262-977-1
EC Name:
Amines, coco alkyl
Cas Number:
61788-46-3
IUPAC Name:
61788-46-3
Details on test material:
- Name of test material (as cited in study report): Cocoalkylamine

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution was prepared with natural river water. Dispersion carried out with: 30 min ultrasound at 40 °C

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: Green algae
- Strain: Desmodesmus subspicatus CHODAT SAG 86.81
- Source: The algae culture was optained from the Institut of plant physiology, University of Göttingen, Germany;
- Method of cultivation: Fresh stock solutions were prepared every month on Z-agar. Light intensity amounted 35-70 µE/m²

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
91.3 mg CO3/L
Test temperature:
23 ± 2 °C
pH:
8.08 - 8.15
Nominal and measured concentrations:
Nominal concentrations: control, 0.03, 0.06, 0.13, 0.25, 0.5 and 1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, fill volume: 250 mL-Erlenmayer-flasks with 100 mL test volume
- Initial cells density: 9000-10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- Standard medium used: Yes


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A natural river water of agricultural backround, middle reach of the river "Böhme", Lower-Saxony was used.
Test water parameter:
pH: 8.2
Conductivity [μS/cm]: 397
DOC [mg C/L]: 7.3
TOC [mg C/L]: 9.9
Ammonium-N [mg N/L]: 0.141
Nitrate-N [mg N/L]: 2.38
o-Phosphate-P [mg P/L]: 0.031
Total Phosphate [mg P/L]: 0.128
Suspended Matter [mg/L]: 17.4
Total Hardness [mg CO3/L]: 91.3
- Culture medium different from test medium: yes
- Intervals of water quality measurement: cell density was measured every 24 hours. Temperature was measured permanently and the pH value was determinated at the beginning and the end of the test.


OTHER TEST CONDITIONS
- Photoperiod: Permanent illumination (24h)
- Light intensity and quality: 60-120 µE/m²*s



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter and microscopic evaluation
Reference substance (positive control):
yes
Remarks:
potassium dicromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.06 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
0.13 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.08 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.03 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
0.06 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass

Any other information on results incl. tables

After 72 h algae were transferred from the nominal dosage levels 0.13 -1.0 mg/L to fresh untreated medium and allowed to grow for further 5 -11 days under test conditions. The test item effect was observed to be reversible at these concentration levels (exept one of three replicates of the highest test concentration of 1 mg/L, where no growth could be detected with 11 d of incubation).

Table 1: Concentration of the test item:

Nominal test item conc.

[mg/L]

 

Meas. conc. [mg/L]after 0 h

Meas. conc. [mg/L]after 3 d

20.00

16.1

12.9

1.00

(18.4)

< detection limit

Control

< detection limit

< detection limit

Applicant's summary and conclusion

Conclusions:
In this study Cocoalkylamine was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus at concentrations > 0.06 mg/L (biomass growth) and > 0.13 mg/L (growth rate). The EC50 values with 95 % confidence intervals for inhibition of biomass growth (EbC50) and specific grothw rate (ErC50) after 72h were 0.08 (0.07 - 0.09) and 0.16 (0.15 - 0.18) mg/L, respectively.
Inhibition effects were found to be reversible up to 1.0 mg/L (all effect levels are given as nominal concentrations)
Executive summary:

In this study Cocoalkylamine was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus at concentrations > 0.06 mg/L (biomass growth) and > 0.13 mg/L (growth rate). The EC50 values with 95 % confidence intervals for inhibition of biomass growth (EbC50) and specific grothw rate (ErC50) after 72h were 0.08 (0.07 - 0.09) and 0.16 (0.15 - 0.18) mg/L, respectively.

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