Benzenepropanenitrile, 2-chloro-.beta.-oxo-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11-13 September 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted in GLP compliance and in accordance with internationally acknowledged guidelines for testing of chemicals.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Chlorobenzoylacetonitril

IUPAC Name:

Chlorobenzoylacetonitril

Test material form:

other: solid

Details on test material:

- Name of test material (as cited in study report): Chlorobenzoylacetonitril
- Physical state: solid
- Analytical purity: > 99%
- Purity test date: 22 Dec 1999
- Lot/batch No.: 46
- Storage condition of test material: stored at 4°C in original container

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: daphnia magna
- Strain: straus, clone 5
- Source: Umweltbundesamt, Berlin, Germany
- Age at study initiation: less than 23.5 hrs


ACCLIMATION
- Acclimation period: 23.5 hrs
- Acclimation conditions (same as test or not): similar conditions

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

2.5 mmol/L as CaCO3

Test temperature:

21°C

pH:

7.7-8

Dissolved oxygen:

>= 8.3 mg/L

Nominal and measured concentrations:

4.6, 10, 21, 42 and 100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 100mL glass beakers
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deoinized water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:no
- Photoperiod: 16h light:8h dark
- Light intensity: 370 Lux


TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 4.6, 10, 12 and 42 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

9.4 mg/L

Nominal / measured:

nominal

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

21 mg/L

Nominal / measured:

nominal

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48h NOEC was determined to be 46 mg/L. The 48h EC 50 was determined to be 9.4 mg/L.