2-[(o-nitrophenyl)azo]-p-cresol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-[(o-nitrophenyl)azo]-p-cresol

EC Number:

215-863-0

EC Name:

2-[(o-nitrophenyl)azo]-p-cresol

Cas Number:

1435-71-8

Molecular formula:

C13H11N3O3

IUPAC Name:

4-methyl-2-[(2-nitrophenyl)diazenyl]phenol

Details on test material:

- Name of test material (as cited in study report): TKA 40136, Monoazo 3113
- Physical state: Red-brownish solid
- Analytical purity: > 95%
- Lot/batch No.: Pa 369
- Storage condition of test material: At room temperature in the dark
- Expiry date: 1997-11-1

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling: During the final test duplicate samples were taken from 0 mg/L, 10 mg/L and the acetone-control for analysis. Sampling frequency: At t= 0 h and at t= 24 h (10 ml from the approx. centre of the test vessels).
- Storage: The samples were stored in a deep-freeze.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Acetone was used as a presolvent to prepare stock solutions.
- Chemical name of vehicle: Acetone
- Concentration of vehicle in test medium: 0.117 mL/L
- Evidence of undissolved material: Yes

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, The Netherlands
- Length at study initiation: Range-finding test: 2.91 ± 0.26 cm, final study: 2.75 ± 0.34 cm
- Weight at study initiation: Range-finding test: 0.74 ± 0.17 g , final study: 0.76 ± 0.21 g
- Feeding during test: No feeding 24 hours prior to the test and during the total test period.

ACCLIMATION
- Acclimation period: At least 12 days
- Type of food: Daily feeding with Trouvit and Artemia
- Mortality: < 5%

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

250 mg/L CaCO3

Test temperature:

20.2-20.6 °C

pH:

8.1

Dissolved oxygen:

7.1-9.6 mg/L

Nominal and measured concentrations:

Nominal: 10 mg/L
Measured:
Spectrophotometric method: 0.58-8.7 mg/L
HPLC method: 1.3-6.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 8 liter glass vessels
- Renewal rate of test solution: Every 24 h
- No. of organisms per vessel: 7
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- No. of vessels per vehicle control: 1
- Biomass loading rate: 0.89 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Photoperiod: 16h daily

EFFECT PARAMETERS MEASURED: Mortality

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1.0 and 10 mg/L
- Results used to determine the conditions for the definitive study: No mortality observed in the highest concentration

Reference substance (positive control):

yes

Remarks:

Pentachlorophenol

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: 0
- Any observations that might cause a difference between measured and nominal values: Precipitation led to a significant decrease of the desired concentration.
- Effect concentrations exceeding solubility of substance in test medium: Yes

Results with reference substance (positive control):

- LC50 = 0.26 mg/L

Applicant's summary and conclusion

Conclusions:

The measured concentrations were clearly lower than the nominal concentration and the LC50 value exceeds the water solubility of the tested substance.