4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline 202 with GLP compliance

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

500-707-7

IUPAC Name:

500-707-7

Constituent 2

Reference substance name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

EC Number:

500-070-7

EC Name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Cas Number:

30583-72-3

Molecular formula:

(C15H28O2.C3H5ClO)x

IUPAC Name:

4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane

Constituent 3

Reference substance name:

Cyclohexanol, 4,4'-(1-methylethylidene)bis-, polymer with 2-(chloromethyl)oxirane

IUPAC Name:

Cyclohexanol, 4,4'-(1-methylethylidene)bis-, polymer with 2-(chloromethyl)oxirane

Details on test material:

As per IUCLID Sections 1.1. 1.2. and 4.1.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

Treatment solutions were prepared by adding the appropriate amount of test substance to the treatment vessels and bring to volume with synthetic fresh water followed by mixing for approximately two hours and 45 minutes.. The final test substance concentration were 10, 20, 40, 80, and 160 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnids were maintained in the Stillmeadow culture laboratory and were no greater than 24 hr old at study start. Daphnia cultures wer e maintained in 250 ml beakers with 200 ml of synthetic fresh water.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Test conditions

Hardness:

225 mg/l CaCO3

Test temperature:

21-22 degrees C

pH:

6.9

Dissolved oxygen:

60-105%

Salinity:

No data

Nominal and measured concentrations:

10, 20, 40, 80 and 160 mg/L nominal

Details on test conditions:

Triplicate cultures of ten daphnids per culture were established for each treatment group. The cultures were maintained at 21-22 C with a 16 hr light/8 hr dark cycle. At 24 and 48 hr following dosing the cultures were observed for immobility and the number of mobile daphnids recorded.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Remarks:

based on immobility.

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% Confidence Limits were 15.8-21.8 mg/L.

Details on results:

Complete immobility was observed in the 40, 80 and 160 mg/L cultures at 48 hr. The percent mobility was 40% in the 20 mg/L cultures at 48 hr. The estimated EC50 value at 24 hr was 28.3 mg/L with 95% Confidence limits of 25-32 mg/L The NOEC at 24 hr was 20 mg/L. The estimated EC50 value at 48 hr was 18.3 mg/L with 95% Confidence Limits of 15.8-21.8 mg/L. The 48 hr NOEC was 10 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of the test substance to Daphnia increased over the 48 hr treatment period. The EC50 value at 24 hr was 28.3 mg/L and the 48 hr EC50 value was 18.3 mg/L. The 24 hr NOEC was 20 mg/L and the 48 hr NOEC was 10 mg/L.

Executive summary:

The toxic effect of the test substance, 4,4'-Isopropylidenedicyclohexanol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, based on immobility was evaluated in an O.E.C.D. 202 test guideline Daphnia study. The EC50 value at 24 hr was 28.3 mg/L and the 48 hr EC50 value was 18.3 mg/L. The 24 hr NOEC was 20 mg/L and the 48 hr NOEC was 10 mg/L.