Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-218-2 | CAS number: 4252-78-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study according to international accepted guidelines but not GLP compliant.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.6 (Degradation: Chemical Oxygen Demand)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.5 (Degradation: Biochemical Oxygen Demand)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidence on the Application of the CLP Criteria
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,2',4'-trichloroacetophenone
- EC Number:
- 224-218-2
- EC Name:
- 2,2',4'-trichloroacetophenone
- Cas Number:
- 4252-78-2
- Molecular formula:
- C8H5Cl3O
- IUPAC Name:
- 2-chloro-1-(2,4-dichlorophenyl)ethan-1-one
- Test material form:
- solid: crystalline
- Details on test material:
- Test item: DICHLOROPHENACYL CHLORIDE (CAS 4252-78-2)
Batch number: T-5427
Colour: beige
Physical state: solid, crystalline powder
Active Ingredient Content (GC): 98.9 %
Storage: on cool and dry place, protected from light
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- 250.0 ml inoculum culture with 1.0 g/l dry substance concentration was made by mixing 20.3 ml stock culture with 229.7 ml mineral medium.
- Duration of test (contact time):
- 5 d
Initial test substance concentration
- Initial conc.:
- 50 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- other: Pressure (The liberated carbon dioxide is absorbed by sodium hydroxide so the biological oxidation generates vacuum wich is registered by the measuring heads.)
- Details on study design:
- This study was performed to evaluate the degradability of Dichlorophenacyl Chloride in an aerobic mixed bacterial culture on the base of quotient of biochemical oxygen demand (BOD) and chemical oxygen demand (COD). The test was carried out in three steps. The degradability was calculated from the quotient of BOD5 and COD according to Guidance on the Application of the CLP Criteria.
For the tests a stock solution of test item was made by dissolving 50.0 mg test item in 1 000 ml mineral medium. The nominal concentration of this stock solution was 50 mg/l.
The chemical oxygen demand was determined by the standard dichromate method. Three replicates were measured in the test.
The biochemical oxygen demand of test item is determined by the OxiTop® system. In this test the test solutions are inoculated with mixed aerobe bacterial culture and poured into 500 ml dark glass bottles. The vessels are placed into incubator at 20 ± 2 °C and continuously stirring with magnetic stirrer. The test duration is 5 days. N-Allylthiourea is added to each test vessel for inhibiting of nitrification.
Results and discussion
% Degradationopen allclose all
- Parameter:
- other: values of vacuum
- Value:
- 7.4
- Sampling time:
- 1 d
- Parameter:
- other: values of vacuum
- Value:
- 7
- Sampling time:
- 2 d
- Parameter:
- other: values of vacuum
- Value:
- 7.4
- Sampling time:
- 3 d
- Parameter:
- other: values of vacuum
- Value:
- 8
- Sampling time:
- 4 d
- Parameter:
- other: values of vacuum
- Value:
- 8.4
- Sampling time:
- 5 d
BOD5 / COD results
BOD5 / CODopen allclose all
- Parameter:
- COD
- Value:
- 2.474 g O2/g test mat.
- Parameter:
- BOD5
- Value:
- 0.209 g O2/g test mat.
- Parameter:
- BOD5*100/COD
- Value:
- 8.4 other: %
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The BOD(5days)/COD ratio is less than 0.5. In this Degradability Test for “Dichlorophenacyl Chloride” (CAS No. 4252-78-2) the results indicated that the test item is not rapidly degradable in the aquatic environment
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
