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EC number: 934-956-3 | CAS number: 1335203-17-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-03-14 to 2002-03-19
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented guideline study. The substance was identified by a statement from the manufacturer. No analytical monitoring.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: "Guideline for measuring the acute toxicity of offshore chemicals to juvenile marine fish, the turbot Scophthalmus maximus and the shepshead minnow Cyprinodon variegatus", based on OECD guideline for Testing of Chemicals n°203 (17/07/92)
- Deviations:
- no
- Principles of method if other than guideline:
- guideline study
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, <2% aromatics
- EC Number:
- 927-632-8
- IUPAC Name:
- Hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, <2% aromatics
- Reference substance name:
- DEV 2001/37
- IUPAC Name:
- DEV 2001/37
- Details on test material:
- - Name of test material (as cited in study report): DEV 2001/37
- Substance type: Petroleum product, UVCB
- Physical state: Colourless liquid
- Analytical purity: 100% Commercial product
- Stability under test conditions: stable
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No test surrogate material is tested
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable (no analytical monitoring)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water accomodation fractions
WAF of different loadings were prepared in 10 L glass flasks by mixing different amounts of test substance with dilution water (natural seawater with a salinityof 32-34 per thousand filtered on a gravel filter to 50-70µm) for 20 hrs. The mixture was sedimented for minimum 2.5 hrs before the WAF were siphoned into the individual test vessels.
- Controls: Dilution water without test substance
Test organisms
- Test organisms (species):
- other: Scophthalmus maximus
- Details on test organisms:
- TEST ORGANISM
- Common name: Turbot
- Source: Stolt Sea Farms, Oye, Norway
- Age at study initiation: Juvenile
- Length at study initiation: 40-60 mm.
- Feeding during test: no feeding
ACCLIMATION
- Acclimation conditions: continuous flow of natural gravel filtered sea water at 10 +/- 3°C and salinity of 32-34 per thousand under normal light intensity (40 W tungsten lamps) at 16:8h L:D cycle.
- Type of food: commercila fish fry feed
- Feeding frequency: every 3 days
Study design
- Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No post exposure observation
Test conditions
- Hardness:
- Not relevant (seawater study)
- Test temperature:
- 15 +/- 1.5 °C
- pH:
- 7.80 - 8.03
- Dissolved oxygen:
- 85 - 98% saturation
- Salinity:
- 45.0 - 45.5 mS
- Nominal and measured concentrations:
- Nominal concentrations (based upon loading rate): 27, 66, 164, 410, 1028 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: White polyethylene vessels, 35 L, filled with 10 L WAF (test) or dilution seawater (control)
- Aeration: yes
- Renewal rate of test solution: every 48 hrs
- No. of organisms per vessel (test): 7
- No. of organisms per vessel (control): 10
- No. of organisms per vessel (sensitivity control): 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per sensitivity control (replicates): 1 per concentration
- Biomass loading rate: test 2.91 mg/L; control 1.38 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Natural seawater with a salinity of 32-34 per thousand filtered in a gravel filter to 50-70 µm.
- Intervals of water quality measurement: every 48 hrs
OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 40 W tungsten lamps
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.5 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 1 028 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Other adverse effects control: None - Results with reference substance (positive control):
- - Results with reference substance valid
- mortality: 20% after 96hr exposure to 0.53 mg/l 3,5-dichlorophenol; 100% mortality after 24hr exposure to 0.67 mg/l 3,5-dichlorophenol - Reported statistics and error estimates:
- No calculations possible
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Test acceptability:
- Temperature in the exposure vessels during 96 hrs of exposure was 15.0 +/- 0.7 °C.
- The fish in the control series showed normal swimming behaviour showing a cumulative immobilization/mortality of 0% after 96 -hr exposure.
- Saturation of oxygen in the control series and the highest concentration of the test did not fall below 85% during the exposure.
- The fish in the sensitivity control series exposed to 0.53 mg/L of 3.5 -dichlorophenol behaved lethagic but with normal swimming behaviour and a cumulative immobilisation/mortality of 20% after 96 -hr exposure. A second sensitivity control series exosed to 0.67 mg/l of 3,5 -dichlorophenol behaved lethargic after 24 hr exposure with a cumulative immobilization/mortality of 100% after 48 hr exposure.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- -sensitivity of the fish exposed to 0.6 mg/l of 3,5-dichorophenol in the range of 20-80% immobile animals; immobilisation in the control series <= 10% after 96-hr exposure; saturation of oxygen greater than 60%; temperature in the range 15+/-1.5°C
- Conclusions:
- 96h-LL50 > 1028 mg/L (based on nominal loading rate)
- Executive summary:
An acute toxicity test was conducted on juveniles of the marine fish Scophthalmus maximus (Turbot). The test substance concentrations (nominal loading rates) ranged from 27 to 1028 mg/L. Test solutions were prepared as individual water accomodated fractions (WAF). Control and dilution water were natural seawater of 32 -34 0/00 salinity filtered through a gravel filter to 50 -70 µm. Test substance was mixed in dilution water for 20 hrs and the mixture was allowed to settle for a minimum of 2.5 hr prior to drawing off the aqueous solution for testing. Test vessels were 35 -L white polyethylene vessels, each containing 10 L of test solution. In the test, 5 concentrations were tested in a geometric series: 27, 66, 165, 410, and 1028 mg/L. Two replicates each containing 7 fishes (biomass loading rate: 2.91 g/L) for the testing and 1 replicate containing 10 fishes (biomass loading rate: 1.38 g/L) for the control were used.
Dissolved oxygen was >60% saturation. The pH was 7.80 - 8.03. The temperature was 15.0 +/- 0.7 °C.
Concentration (mg/L)
Day 0 (initiation)
Day 4 (96 hrs)
Temperature (°C)
Salinity (mS)
% O2
pH
Temperature (°C)
Salinity (mS)
% O2
pH
Control
15.1
45.0
96
8.01
15.7
45.3
87
7.86
27
-
45.0
93
8.01
-
45.4
90
7.82
66
-
45.1
92
8.02
-
45.3
89
7.81
165
-
45.0
91
8.01
-
45.2
90
7.81
410
-
45.0
90
8.03
-
45.4
91
7.82
1028
-
45.0
90
8.04
-
45.5
92
7.83
Test organisms were obtained from a fish farm (Stolt Sea Farms, Oye, Norway). At the test start, the organisms were 40 - 60 mm long juveniles.
No deviations from the protocol were noted.
Analytical monitoring was not performed. Therefore the results are based on the loading rate.
Nominal loading (mg/L)
Number of fish
Initial
Immobilised/dead
24h
48 h
72 h
96 h
Cumulative
Control
10
0
0
0
0
0
27
7 x 2
0
0
0
0
0
66
7 x 2
0
0
0
0
0
165
7 x 2
0
0
0
0
0
410
7 x 2
0
0
0
0
0
1028
7 x 2
0
0
0
0
0
96h-LL50>1028 mg/L (based upon nominal loading rate).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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