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Ecotoxicological information

Toxicity to other above-ground organisms

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Administrative data

Endpoint:
toxicity to other above-ground organisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Effects of dietary zinc level and an inflammatory challenge on performance and immune response of weanling pigs
Author:
Guo-Jun S, Dai-Wen C, Ke-Ying Z, Yu B
Year:
2009
Bibliographic source:
Asian-Australasian J. Anim Sci. 22 1303–1310.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Dietary toxicity study in pigs
GLP compliance:
no
Remarks:
peer-reviewed publication without information on GLP

Test material

Constituent 1
Chemical structure
Reference substance name:
Zinc sulphate
EC Number:
231-793-3
EC Name:
Zinc sulphate
Cas Number:
7733-02-0
Molecular formula:
H2O4S.Zn
IUPAC Name:
zinc sulfate
Details on test material:
- Name of test material (as cited in study report): zinc sulphate (ZnSO4.7H2O)

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Tissue samples were prepared for analysis by wet digestion with HNO3 and HClO4, The dried, fat-free phalanges were ashed at 550°C for 48 h, and bone samples were then dissolved in 5 ml of 6 N HC1 and diluted appropriately for Zn analysis.

Test organisms

Test organisms (species):
other wild mammal species: pigs
Details on test organisms:
TEST ORGANISM
- Age at test initiation: weaned at 28 d
-weight: 6.76 ± 0.47kg
- Kept according to standard practices: yes (Sichuan Agricultural University Animal Care and Use Committee)

Study design

Study type:
laboratory study
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
0 days

Test conditions

Test temperature:
25-28°C
Humidity:
not reported
Photoperiod and lighting:
12 h light:12 h dark were
Details on test conditions:
Housing: 1.28×1.28 m pens (cycle of 12 h light:12 h dark)
Daily food intake per body weight (kg diet /day/ kg bw): 334 (Control), 332 (60 Zn mg/kg), 337 (120 Zn mg/kg)
Control: Basal diet without Zn supplementation
Concentration in control diet (mg Zn/kg diet): 36.75
No of animals (M/F) per dose: SIx replicates per treatment with
four pigs per pen
Nominal and measured concentrations:
dose (mg Zn/kg diet, added): 0, 60, 120 (nominal values)

Results and discussion

Effect concentrations
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
>= 120 mg/kg diet
Nominal / measured:
nominal
Conc. based on:
element (total fraction)
Remarks:
Zn
Basis for effect:
growth
Remarks:
average daily growth
Details on results:
no significantly different effects of different Zn concentrations

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
An unbounded NOEC of ≥120 mg Zn/kg diet (added as ZnSO4) was observed fro growth of pigs.