p-toluonitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

16. July - 02. September

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

p-toluonitrile

EC Number:

203-244-8

EC Name:

p-toluonitrile

Cas Number:

104-85-8

Molecular formula:

C8H7N

IUPAC Name:

p-toluonitrile

Details on test material:

- Name of test material (as cited in study report): 4-Toluonitrile

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

nat applicable

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
-Strain: Dahnia magna (Straus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Test conditions

Hardness:

no data

Test temperature:

19.3 - 21.7°C

pH:

7.78 - 8.13

Dissolved oxygen:

8.4 - 9.0 mg/l

Salinity:

no data

Nominal and measured concentrations:

- 4.27; 9.39; 20.7; 45.5 mg/l

Details on test conditions:

four 100ML glass beakers per concentration, each filled with 50 ML, one additional replicate for measurements of physical-chemical propertiers

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

>= 9.39

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 9.39

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

>= 15.1

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 d

Dose descriptor:

EC50

Effect conc.:

>= 15.4

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

According to the OECD 202 guidline this study can be regarded as valid, since
- in the control not more than 10% of animals were immobilised or showed any other signs of diseases, for example discoloration or unusual behaviour such as trapping on surface
- the dissoved oxygen concentration was >/- 3 mg/l at the end of the test in control and test vessels

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

no statistics reported

Any other information on results incl. tables

Table 1: Results of the main test, 24 h values

Concentration Test Item	Control	4.27	9.39	20.7	45.5	100
		[mg/L]
	Immobilized daphnids after 24 h
Group 1	0	0	0	5	5	5
Group 2	0	0	0	5	5	5
Group 3	0	0	0	5	5	5
Group 4	0	0	0	3	5	5
∑	0	0	0	18	20	20
%	0	0	0	90	100	100

 

Table 2: Result of the main test, 48 h values

Concentration Test Item	Control	4.27	9.39	20.7	45.5	100
		[mg/L]
	Immobilized daphnids after 48 h
Group 1	0	0	0	5	5	5
Group 2	0	0	1	4*	5	5
Group 3	0	0	0	2*	5	5
Group 4	0	0	0	5	5	5
∑	0	0	1	16	20	20
%	0	0	5	80	100	100

*some daphnids recovered

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

- in the control not more than 10% of animals were immobilised or showed any other signs of diseases, for example discoloration or unusual behaviour such as trapping on surface;

Conclusions:

According to the results of the test, the EC50 (48 H) for immobilisation was determined to be 15,4 mg/L (nominal).

The corresponding NOEC (48 h) was determined to be 9.39 mg/L (nominal):

Executive summary:

The toxicological endpoints were evaluated using nominal concentrations of the test item.

The total hardness of the unreated control was determined to be 12°dH (214 mg/L CaCO3); the mean pH-value of the untreated control was determined to be 7.95 +/- 0.18 (Std. Dev.), the mean temperature of the control and all test item concentrations was measured to be 20.2 +/- 0.9°C (Std. Dev.) and the mean oxygen concentration was determined to be 8.7 +/- 0.2 mg/L (Std. Dev.).