5-[(4-chloro-2-nitrophenyl)azo]-1-ethyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental starting date: 27 February 2015; Experimental Completion date: 03 April 2015; Study completion date: 03 May 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

flask method

Specific details on test material used for the study:

Identification: FAT 36091/I TE Lot.20131205lab
Appearance: Yellow powder

Water solubility:

3.9 mg/L

Conc. based on:

test mat.

Temp.:

20 °C

Remarks on result:

other: Without correction of the purity.

Individual Results:

Preliminary Visual estimation of the water solubility

Appearance of the test mixtures in the preliminary test:

Amount of the test item in mg	Total volume in ml	Appearance of mixture
10	10	undissolved
10	50	undissolved
10	100	undissolved

The preliminary visual experiments showed that the water solubility of the test item is below 100 mg/l.

Flask-method

Water solubility of the etst item- experiment A to D

Experiment	A	B	C	D (Blank)
Amount of the test item in mg	500	500	500	/
Stirring time at 30°C (h)	24	48	72	72
Measured concentration (micrgr/l)	0.77	0.73	0.83	/
Aliquot	2/10 ml	2/10 ml	2/10 ml	2/10 ml
Water solubility (g/l)	3.9	3.7	4.1	/

Average experiments A to C: 3.9 mg/l

Standard addition for recheck

Standard	Measured concentration (microgr/ml)	% Recovery
Sample 3+ 1.16 microgr test item	2.02	101.5

The recovery of 101.5 % indicates a sufficient accuracy of the analytical method.

Conclusions:

The water solubility cs of the test item at a temperature of 20 °C was determined to (without correction for the purity): cs = 3.9 mg/l The test substance is slightly soluble.

Executive summary:

The water solubility of the test substance was determined in a study conducted according to OECD Guideline 105 and EU Method A.6. For the main test three flasks were prepared with about 500 mg test item and 5 ml water. The batches were stirred for different times at 30 °C (experiment A for 24 h, experiment B for 48 h, experiment C for 72 h) in tightly closed flasks. After equilibration (further stirring for 24 h at 20 °C) a saturated solution with a precipitate of the test item could be observed. Aliquots were taken and centrifuged (30 minutes with 5000 rpm at 20 °C). Subsequently, the solution was filtered over 0,20 μm microfilters. The solution is yellow colored and analyzed by VIS spectroscopy (443 nm). Another experiment was performed according to the procedure of experiment without the test item (blank). The water solubility cs of the test item at a temperature of 20°C was determined to (without correction for the purity): cs = 3.9 mg/l. The test substance is slightly soluble.

Description of key information

The water solubility of the test substance was determined in a study conducted according to OECD Guideline 105 and EU Method A.6. For the main test three flasks were prepared with about 500 mg test item and 5 ml water. The batches were stirred for different times at 30°C (experiment A for 24h , experiment B for 48h , experiment C for 72h ) in tightly closed flasks. After equilibration (further stirring for 24h at 20 °C) a saturated solution with a precipitate of the test item could be observed. Aliquots were taken and centrifuged (30 minutes with 5000 rpm at 20 °C). Subsequently, the solution was filtered over 0.20 μm micro filters. The solution is yellow colored and analyzed by VIS spectroscopy (443 nm). Another experiment was performed according to the procedure of experiment without the test item (blank). The water solubility cs of the test item at a temperature of 20 °C was determined to (without correction for the purity): cs = 3.9 mg/l The test substance is slightly soluble.

Key value for chemical safety assessment

Water solubility:

3.9 mg/L

at the temperature of:

20 °C