2-bromo-N-[4-chloro-2-(2-chlorobenzoyl)phenyl]acetamide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23.04-05.10.2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-bromo-N-[4-chloro-2-(2-chlorobenzoyl)phenyl]acetamide

EC Number:

226-844-1

EC Name:

2-bromo-N-[4-chloro-2-(2-chlorobenzoyl)phenyl]acetamide

Cas Number:

5504-92-7

Molecular formula:

C15H10BrCl2NO2

IUPAC Name:

2-bromo-N-[4-chloro-2-(2-chlorobenzoyl)phenyl]acetamide

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

4.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Remarks on result:

other: not readily biodegradable

BOD5 / COD results

Results with reference substance:

The percentage degradation of the reference substance has reach the pass level of 60% on day 6.

Any other information on results incl. tables

The degradation in the toxicity control indicated that the substance concentration of the test item had no inhibitory effect.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The ready biodegradation of BDB in an aerobic aqueous medium was tested by the Closed Bottle Test according to the guideline 92/69/EEC, part C.4-E. Mineral medium containing a known concentration of the test item was inoculated with microorganisms from. The eflluent of a treatment plant (domestic sewage) and incubated in the dark over a 28 day period. Degradation was followed by oxygen evolution twice a week. The amount of oxygen taken up by the microbial population during biodegradation of the test item is expressed as a percentage of the theoretical oxygen demand of the test item.
Throughout the experiment no marked values of oxygen uptake were measured. The result of a toxicity control showed that the concentration of test item tested had no inhibitory effect to the inoculum.
The pass level for ready biodegradation ( 60 per cent degradation) was not reached. Thus in the test for ready biodegradability the test item seems to be not readily biodegradable.