Fatty acids, rape-oil, Me esters

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: ISO (1990) proposal to TC147/SC5/WG2

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids, rape-oil, Me esters

EC Number:

287-828-8

EC Name:

Fatty acids, rape-oil, Me esters

Cas Number:

85586-25-0

Molecular formula:

Unspecified

IUPAC Name:

methyl (11Z)-icos-11-enoate; methyl (13Z)-docos-13-enoate; methyl (9E)-octadec-9-enoate; methyl (9Z,12Z)-octadeca-9,12-dienoate

Details on test material:

- Name of test material (as cited in study report): Rapeseed oil fatty acid methyl ester
- Physical state: yellowish liquid
- Analytical purity: 100%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

not specified

Test organisms

Test organisms (species):

other: Acartia tonsa

Study design

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

19.3 - 20.4

pH:

8.17 - 8.32

Dissolved oxygen:

94 - 98% saturation

Salinity:

not specified (filtered seawater used)

Nominal and measured concentrations:

Nominal: 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass crystallising dishes containing 50 mL test solution
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: filtered and ultra violet treated seawater (Scapa Flow, Orkney)

EFFECT PARAMETERS MEASURED: Mortality was recorded after 24h and 48h test duration.

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1, 10, 100 and 1000 mg/L

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The marine copepod Acartia tonsa showed a 48h-EC50 value > 1000 mg/L in a toxicity test performed according to GLP and a test design, which is similar to OECD guideline 202.