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EC number: 262-134-8 | CAS number: 60270-33-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
48 h-EC50 = 1.16 mg/L, analysed test concentrations of the untreated solution (Daphnia magna; OECD guideline 202, GLP, RL1)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.16 mg/L
Additional information
The 48-hr-acute toxicity of DIMAPDO lactate to Daphnia magna was studied under semi-static conditions according to OECD guideline 202 (April 13, 2004) and Daphnia sp., Acute Immobilization Test stipulated in the "Testing Methods for New Chemical Substances" (November 21, 2003; No. 1121002, Pharmaceutical and Food Safety Bureau, MHLW). Daphnids were exposed to control, and test chemical at measured concentrations of untreated test solution of 0.245, 0.497, 0.999, 1.99, 3.74, 7.82 mg test substance/L for 48 hr. The analysed test concentrations of the untreated solution (analysis of dispersed + dissolved test substance) and not of the centrifuged solution (analysis only of dissolved test substance) were chosen because in the OECD guideline 201 study there was adiscontinuity in the analysis of the concentrations of the centrifuged test solution and therefore the calculated values for the centrifuged test media were not valid. Additionally in the OECD guideline 203 study the examination of the dead fish revealed that the dispersed test material had adhered to the gill filaments indicating physical toxicity of the dispersed test material particles that may be also a factor in toxicity to daphnia magna.
Mortality/immobilization and sublethal effects were observed daily. The 48-hour EC50 was 1.41 mg test substance/L. The 48-hr NOEC based on immobilization was 0.497 mg test substance/L.Because the study was conducted with lactic acid salt of DIMAPDO the EC50 and NOEC values of DIMAPDO based on % immobilization were calculated as 1.16 mg/L and 0.41 mg/L active ingredient.This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age: less than 24 hours
Test Type: Semi-static (daily renewal)
NOEC: 0.41 mg a.i./L
EC50: 1.16 mg a.i./L 95% C.I.: 0.924 to 1.452 mg a.i./L
Endpoint Effected: immobilisation
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