1-amino-4-({3-[(4-chloro-6-{[(3 or 4)-sulfophenyl]amino}-1,3,5-triazin-2-yl)amino]-2,4,6-trimethyl-5-sulfophenyl}amino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid, lithium sodium salts

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

The main structure of the read-across substance is the same of the CJ304, only the salt form is different. However, this difference does not influence the toxicity of the CJ304. CJ304 contains Li/Na salt form and the read-across substances contains only Na salt. This will not lead to difference on the toxicity results.

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Version / remarks:

Method B-21

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

trilithium(1+) trisodium 1-amino-4-{[3-({4-chloro-6-[(3-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2,4,6-trimethyl-5-sulfonatophenyl]amino}-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate 1-amino-4-{[3-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2,4,6-trimethyl-5-sulfonatophenyl]amino}-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Molecular formula:

Not applicable; this UVCB substance contains: C32H23ClN7O11S3.xLi.yNa, (x + y) = 3; 0 < (x,y) < 3 with 834.0 < MW < 882.1 g/mol (UVCB substance), C38H28N8O14S4.xLi.yNa, (x + y) = 4; 0 < (x,y) < 4 with 976.6 < MW < 1040.8 g/mol (UVCB substnace), and traces of NaCl and Na2SO4.

IUPAC Name:

trilithium(1+) trisodium 1-amino-4-{[3-({4-chloro-6-[(3-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2,4,6-trimethyl-5-sulfonatophenyl]amino}-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate 1-amino-4-{[3-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2,4,6-trimethyl-5-sulfonatophenyl]amino}-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Specific details on test material used for the study:

Name: FAT 41001/F
Purity: >90%

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

activated sludge

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

pH:

7.4 - 7.6

Dissolved oxygen:

4.7 - 5.1

Nominal and measured concentrations:

1, 3.2, 10, 32, 100 and 320 mg/L

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Results with reference substance (positive control):

3h IC50 for 3,5 - Dichlorophenol = 11 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The IC50 of FAT 41001/F was found to be greater than 320 mg/L.

Executive summary:

A study was performed with FAT 41001/F to determine the toxicity to microorganisms. This test was carried out in accordance to OECD test guideline 209 and CIBA internal method B-21.

The concentration of test substance used: 1, 3.2, 10, 32, 100, 320 mg/L. 3,5 - Dichlorophenol was used as reference substance.

Based on the findings of the study, the 3h IC50 for FAT 21030/A was found to be greater than 320 mg/L. The 3h IC50 of reference substance was determined to be 11 mg/L.