Ethanol, 2-[(3-nitropyrazolo[1,5-a]pyridin-2-yl)oxy]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From September 2014 to February 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well described study performed according to OECD guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Remarks:

Certificate available in the attached report.

Test material

Test material information

Constituent 1

Reference substance name:

2-({3-nitropyrazolo[1,5-a]pyridin-2-yl}oxy)ethan-1-ol

EC Number:

938-829-3

Cas Number:

936103-10-5

Molecular formula:

C9H9N3O4

IUPAC Name:

2-({3-nitropyrazolo[1,5-a]pyridin-2-yl}oxy)ethan-1-ol

Constituent 2

Reference substance name:

2-[(3-Nitropyrazolo[1,5-a]pyridin-2-yl)oxy] ethanol

IUPAC Name:

2-[(3-Nitropyrazolo[1,5-a]pyridin-2-yl)oxy] ethanol

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Test solutions

Details on test solutions:

Test Concentrations:
100 mg test item/L and a control.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Female Daphnia magna, clone 5; 6.0 to 22.5 hours old.
Source: The daphnids introduced in the test were taken from IBACON's in-house laboratory culture.
The daphnids were bred in the laboratories of IBACON under similar temperature and light conditions as used in the test. The cultivation of the parental daphnids was performed in the same reconstituted water as the test water used in the test. The test organisms were not first brood progeny. The daphnids in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of IBACON.

Study design

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Water Hardness: 2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

Test Environment: Controlled environment room
Water temperature: 20 °C

pH:

pH value: 7.7 – 8.2

Dissolved oxygen:

dissolved oxygen concentration: 7.7 – 8.8 mg/L

Details on test conditions:

This study encompassed two treatment groups (one test item concentration at nominal 100 mg/L and one control) each containing 20 individuals.
Photoperiod: 16 h light - 8 h dark.
Light intensity: 240 – 340 lux.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Duration:

24 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

See report attached.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The toxic effect of the test item R0056895A to Daphnia magna was assessed in a static limit test. The 48-hour NOEC was determined to be 100 mg test item/L. The 48-hour LOEC was determined to be > 100 mg test item/L and the 48-hour EC50 value was determined to be > 100 mg test item/L.