Benzyltriethylammonium chloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19/07/2016 - 23/07/2017

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard OECD test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the Zebra Fish Danio rerio .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

GLP compliance:

yes

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Source:Unique Aqua Fish Corner
- Age at study initiation (mean and range, SD):No data
- Length at study initiation (length definition, mean, range and SD):average 3.42 cm
- Weight at study initiation (mean and range, SD):average 0.335 g
- Method of breeding:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Food type:Standard brand feed
- Frequency:once daily

ACCLIMATION
- Acclimation period:7 days
- Acclimation conditions (same as test or not):Fishes, to be used in the study, are kept in the test water for seven days immediately before testing
- Type and amount of food:Standard brand feed
- Feeding frequency:Once in a day up to sixth day of acclimatization
- Health during acclimation (any mortality observed):No mortality

HOUSING:
-Duration of housing:15 days
-Photoperiod-12 hours light and 12 hours darkness
-Oxygen content-7.06 (mg/l)

QUARANTINE (wild caught)
- Duration:96 hrs
- Health/mortality:During last 48 hours = 0 %

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

140.5(mg of CaCO3)

Test temperature:

25°C

pH:

7.14

Dissolved oxygen:

6.66 mg/l

Salinity:

No data

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

Test vessels:The test vessels used were all plastic aquaria having the water capacity of 5liters.

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality and symptoms

NominalConcentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24h			48h		72h		96h
			V1	V2	V1	V2	V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	-	-	-	-	-		-	-	-	-	-	-	-
100	4	4	-	-	-	-	-		-	-	-	-	-	-	-

 

Visible Symptoms: 

NominalConcentration (mg/l)

Number of fish

Symptoms after

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

0 (control)

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

 

Explanation of symptoms:

A:apathy

N:Narcotic-like state

C:Swimming near water surface

M: Slow movements compared to control

NS:No abnormal symptoms 

S:Surfacing behaviour

L:Loss of equilibrium

SM:Shimming movement

H1:Hyperactivity

H2:Hypo activity

B:Bending behavior

F:Fin rot

IS:Invert Swimming

SB:Swimming behaviour

RF:Respiratory Function

P:Pigmentation

 

 

 

 

 

 

pH and oxygen content:

 

NominalConcentration (mg/l)	Number of fish		pH readings after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	7.26		7.27	7.41	7.46	8.11	8.12	8.17	8.19
100	4	4	7.71		7.22	7.92	7.93	8.05	8.06	8.21	8.23

 

NominalConcentration (mg/l)	Number of fish		Temperature (°C) after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	7.1		7.5	7.1	7.6	6.9	6.3	5.4	5.4
100	4	4	6.9		7.2	7.4	7.5	6.2	6.1	6.7	6.9

 

Test temperature

NominalConcentration (mg/l)	Number of fish		Oxygen content (mg/L) after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	22		22	22	22	22	22	22	22
100	4	4	22		22	22	22	22	22	22	22

 

Validity criteria fulfilled:

not specified

Conclusions:

In short term toxicity to fish based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra- Fish Danio rerio was found to be >100mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material. The test material was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.The nominal concentration selected for the experiment were 100 mg/L and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L.

Description of key information

Short term toxicity to fish:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material.The test material was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.The nominal concentration selected for the experiment were 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.The lethal concentrations LC50 was found to be >100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Short term toxicity to fish:

Various studies of short term toxicity to fish from experimental study report and peer reviewed journals for the target chemical were reviewed to summarize the following information:

 

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L. The nominal concentration selected for the experiment were 100 mg/L and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L. Experimental key study report indicate that the Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical .The nominal concentration selected for the experiment were 100 mg/L and Zebra Fish-Danio rerio were exposed to these concentration for 96 hours. The lethal concentrations LC50 was datarmine to be >100 mg/L.

Other supporting study from peer reviewed journal showed short term toxicity study on Fathead Minnows (Pimephales promelas) which was treated with Benzyl triethyl ammonium chloride in a measured concentration of 0, 46.5, 71.5, 110, 170 and 262 mg/l for 96 hours. 50 % mortality was observed at 161 mg/l at confidence limit of 141- 185 mg/l. Loss of schooling behavior, hyperactive and over reactive to external stimuli were observed in treated fish and were darkly colored. Equilibrium loss was not observed prior to death. Therefore, LC50 was considered to be 161 mg/l when Fathead Minnows (Pimephales promelas) treated with test material for 96 hours.

 

Above studies supported by another authorative database as study indicate that acute toxicity to fish test has been carried out to evaluate the ecotoxicological effects of chemical with dose concentration of 100 mg/L, 180 mg/L, 320 mg/L, 560 mg/L and 1000 mg/L. The test organism was Oryzias latipes and the test carried out in semi-static condition throughout the test temperature maintained at 24 +/- 1 degree C. The 96-h LC50 value was observed to be 694 mg/L when exposed to Oryzias latipes with test chemical.

 

Thus on the basis of the above experimental key study results and peer reviewed journals concluded that the target chemical can be non- hazardous to fish and therefore not consider for the further classification as per the CLP criteria.