Tetrasodium 3,3'-[[6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diyl]bis[imino(2-methyl-4,1-phenylene)azo]]bisnaphthalene-1,5-disulphonate

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-08-23 to 1988-02-18

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: national standard method

GLP compliance:

no

Radiolabelling:

not specified

Vehicle:

no

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Length at study initiation (length definition, mean, range and SD): 9.7 cm
- Weight at study initiation (mean and range, SD): 25.2 g
- Lipid content at test initiation (mean and range, SD): 5 %

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Hardness:

no data

Test temperature:

25 °C +/- 1 °C

Dissolved oxygen:

6.5 - 7.0 mg/L (2 mg/l test substance), 6.2 - 6.9 (0.2 mg/L test substance)

Details on test conditions:

TEST SYSTEM
- Test vessel: 100L, made of glass
- Flow rate: 582 L/day
- Dilution water: 4 ml/min; 400 ml/min
- Concentration stock solution: 200 ppm (w/v), 20 ppm (w/v)
- No. of vessels per concentration (replicates): 2
- External desinfection: 10 mg/L chlorotetracycline hydrochloride in an aquarius solution for 24 hours

RANGE-FINDING / PRELIMINARY STUDY
- Results used to determine the conditions for the definitive study: > 1000 mg/L
- 48 h (Oryzias latipes)

Nominal and measured concentrations:

0.2 and 2 mg/L

Reference substance (positive control):

not specified

Lipid content:

5 %

Conc. / dose:

2 mg/L

Type:

BCF

Value:

> 0.6 - < 1.8 L/kg

Conc. / dose:

0.2 mg/L

Type:

BCF

Value:

< 5.5 L/kg

Details on results:

- Behavioural abnormalities: no abnormalities observed

Validity criteria fulfilled:

not specified

Conclusions:

The BCF was 0.6 - 1.8 L/kg at 2 mg/L of Direct Yellow 86.

Executive summary:

According to a national standard method a bioaccumulation study was performed. Fish (cyprinus caprio) were kept in the continuous flow-through bioconcentration test system comprised of two sets of test substance level (0.2 and 2 mg/L) for 6 weeks. During the exposure period, the concentrations of the test substance in the test water and fish were measured periodically, and their ratios were used to calculate the bioconcentration factors (BCF) to evaluate bioconcentration potential of the test substance in fish. A range finding test was performed with the test fish Oryzias latipes for 48 hours with the result > 1000 mg/L. In the BCF study body weight and body length was measured. The length of the fish was 9.7 cm, the weight 25.2 g and the lipid content 5 %. The 100 L glas test vessel has a flow rate of 582 L/day. An external desinfection with 10 mg/L chlorotetracycline hydrochloride in an aquarius solution was made for 24 hours. The test temperature was 25 °C +/- 1 °C.

At end of the test no abnormalities were observed. The BCF was 0.6 - 1.8 L/kg at 2 mg/L and < 5.5 L/kg at 0.2 mg/L test substance.

Description of key information

The BCF was 0.6 - 1.8 L/kg at 2 mg/L of Direct Yellow 86.

Key value for chemical safety assessment

BCF (aquatic species):

1.8 L/kg ww