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EC number: 943-342-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 2016 - August 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP compliant
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Lot/batch No.of test material: hydrogenious 005
- Expiration date of the lot/batch: >3 years, unlimited shelf life
- Purity: > 95%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at ambient temperature
- Stability under test conditions: stable - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Cambridge Sewage Treatment Works, Cowley Road, collected on 05 July 2016
- Pretreatment of sludge: Sieved to 850μm to remove coarse particulates, settled and centrifuged at ~ 4000rpm for ~ 5-10 minutes. The supernatant discarded, sludge re-suspended in mineral media and centrifuged at ~ 4000rpm for ~ 5-10 minutes. This stage repeated once more then the supernatant discarded again and homogenised thoroughly by mechanical stirring (spoon). Dry sludge solid detemined on the pellet produced.
- Concentration of sludge: 7.31% dry sludge solids
- Initial cell/biomass concentration: 0.030 g/l dry sludge solids in test - Duration of test (contact time):
- 28 d
- Initial conc.:
- 19.99 mg/L
- Based on:
- TOC
- Remarks:
- Test vessel 1
- Initial conc.:
- 20.05 mg/L
- Based on:
- TOC
- Remarks:
- Test vessel 2
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium acc. to guideline
- Test temperature: 22 ± 2 °C
- Suspended solids concentration: 0.030 g/l
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 1 wash bottle, filled with soda lime to remove CO2 from the atmosheric air pumped through the system, followed by the test vessel, followed by 2 carbon dioxide traps
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: atmospheric air pumped into the test system
- Measuring equipment: Dissolved Inorganic Carbon (DIC) measured using a Tekmar-Dohrmann Phoenix 8000 (UV-Persulfate Analyser)
- Details of trap for CO2: 200 ml of 0.05 M sodium hydroxid solution
SAMPLING
- Sampling frequency: at days 2, 5, 7, 9, 14, 19, 23, 28, and 29
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: none
- Toxicity control: 1 - Reference substance:
- acetic acid, sodium salt
- Remarks:
- CAS No.: 127-09-3, supplied by Fisher scientific, Lot/batch number: 1280233, expiry date: April 2017, composition and purity: ≥99%
- Test performance:
- Conical flasks of nominal volume 2000ml were filled with 1500ml of inoculated mineral medium. The blanks, reference and test bottles were set up in duplicate, with a single replicate for the toxicity control. Test and reference materials were added to appropriate bottles to a final concentration of 20mg Carbon/l respectively. Atmospheric air was pumped into the test system and scrubbed clean of carbon dioxide by passing over soda lime. The air continued into the test vessel where it collects any evolved carbon dioxide before moving into the carbon dioxide traps, each containing 200ml of 0.05M sodium hydroxide solution. The test solutions were stirred for the duration of the study. The carbon dioxide evolved from each bioreactor flask was trapped in 200ml aliquots of 0.05M sodium hydroxide which was sacrificially sampled at specific time points. On day 28, 2ml of 50% (v/v) hydrochloric acid was added to each bioreactor, which were then aerated overnight, to drive off the remaining carbon dioxide. One last analysis of evolved carbon dioxide was made on day 29. The toxicity control was prepared by adding to the flask the same quantities of carbon as used in each of the respective test and reference material flasks (20mg/l of each). The degradation of the reference material in the toxicity control was determined by using the average test sample value as the “blank” at each time point.
The concentration of carbon dioxide was determined as inorganic carbon (mg/l) and converted to mg carbon in 200ml of sodium hydroxide (concentration x 200/1000). The cumulative quantities of carbon (adjusted for the average cumulative blank) were determined for each of the blank, reference, test and toxicity control vessels. The percentage degradation was calculated for all flasks at each of the time points.
degradation (%) = (mg inorganic carbon (test/reference/toxicity control) – mg average blank) x 100 / mg carbon (test/reference/toxicity control) - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance dibenzylbenzene, ar-methyl derivative, hydrogenated is not readily biodegradable under the conditions of this test.
- Executive summary:
The test substance dibenzylbenzene, ar-methyl derivative, hydrogenated was determined in a Ready Biodegradability – CO2 Evolution test according to OECD Guideline 301B in compliance with GLP. Dibenzylbenzene, ar-methyl derivative, hydrogenated failed to meet the requirements for a pass in this test (≥60% degradation relative to the ThCO2 value) with a maximum of -1% (this dropped to -3% during the study). The inoculum blank should not normally produce more than 40mg/l CO2 (10.9mg/l C) for the test to be valid. A value of 26.9mg/l CO2 (7.3mg/l C) was recorded. The guideline requires that the reference material degrades by at least 60% of its ThCO2 value, a maximum value of 81% degradation of the sodium acetate was achieved on day 29. A toxicity control containing dibenzylbenzene, ar-methyl derivative, hydrogenated and the reference material (both at 20mg/l) was run in parallel. Inspection of the data generated indicates that, whilst dibenzylbenzene, ar-methyl derivative, hydrogenated was not found to be degradable, it was also not inhibitory to the micro-organisms responsible for the degradation of the reference material.
The final conclusion of the study is therefore that dibenzylbenzene, ar-methyl derivative, hydrogenated is not readily biodegradable under the conditions of this test.
Reference
Table: Percentage degradation |
|||||
Time [days] | Test substance | Reference (sodium acetate) |
Toxicity control | ||
vessel 1 | vessel 2 | mean | mean | ||
0 | 0 | 0 | 0 | 0 | 0 |
2 | -1 | 0 | -1 | 29 | 8 |
5 | -3 | -1 | -2 | 48 | 21 |
7 | -3 | -1 | -2 | 54 | 25 |
9 | -4 | -2 | -3 | 58 | 27 |
14 | -4 | -2 | -3 | 64 | 30 |
19 | -1 | -4 | -2 | 64 | 29 |
23 | -2 | -4 | -3 | 66 | 30 |
28 | -2 | -4 | -3 | 67 | 31 |
29 | -1 | -4 | -3 | 70 | 33 |
29 | 1 | -5 | -2 | 81 | 40 |
Description of key information
The test substance dibenzylbenzene, ar-methyl derivative, hydrogenated was determined in a Ready Biodegradability – CO2 Evolution test according to OECD Guideline 301B in compliance with GLP. Dibenzylbenzene, ar-methyl derivative, hydrogenated failed to meet the requirements for a pass in this test (≥60% degradation relative to the ThCO2 value) with a maximum of -1% (this dropped to -3% during the study). The inoculum blank should not normally produce more than 40mg/l CO2 (10.9mg/l C) for the test to be valid. A value of 26.9mg/l CO2 (7.3mg/l C) was recorded. The guideline requires that the reference material degrades by at least 60% of its ThCO2 value, a maximum value of 81% degradation of the sodium acetate was achieved on day 29. A toxicity control containing dibenzylbenzene, ar-methyl derivative, hydrogenated and the reference material (both at 20mg/l) was run in parallel. Inspection of the data generated indicates that, whilst dibenzylbenzene, ar-methyl derivative, hydrogenated was not found to be degradable, it was also not inhibitory to the micro-organisms responsible for the degradation of the reference material. The final conclusion of the study is therefore that dibenzylbenzene, ar-methyl derivative, hydrogenated is not readily biodegradable under the conditions of this test.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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