Registration Dossier
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EC number: 205-778-7 | CAS number: 150-90-3
Endpoint summary
Administrative data
Description of key information
Biodegradation in water
28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test chemical (Experimental study report, 2018). The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item.The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 76.5%. Degradation of Sodium Benzoate exceeds 45.18% on 7 days & 70.48% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid.The BOD28 value of test chemical was observed to be 0.67 mgO2/mg. ThOD was calculated as 0.69 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 97.1%. Based on the results, the test item, under the test conditions, was considered to be readily biodegradable in nature.
Biodegradation in water and sediment
Estimation Programs Interface (2018) prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 29.3% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 8.66 days (208 hrs). The half-life (8.66 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of test chemical in sediment is estimated to be 77.916 days (1870 hrs). Based on this half-life value, it indicates that test chemical is not persistent in sediment.
Biodegradation in soil
The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (2018). If released into the environment, 70.7% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil is estimated to be 17.33 days (416 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.
Additional information
Biodegradation in water
Various experimental key and supporting studies of the test chemical were reviewed for the biodegradation end point which are summarized as below:
In an experimental key study from study report (2018),28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test chemical. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 76.5%. Degradation of Sodium Benzoate exceeds 45.18% on 7 days & 70.48% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.67 mgO2/mg. ThOD was calculated as 0.69 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 97.1%. Based on the results, the test item, under the test conditions, was considered to be readily biodegradable in nature.
For the test chemical, the Biodegradation experiment was carried out for determining biodegradability of test chemical following the OECD Guideline 301 C "Ready Biodegradability: Modified MITI Test (I)" under aerobic conditions (from authoritative database, handbook and secondary source, 2018). In this experiment, non adapted activated sludge was used as test inoculum for the study at 30 mg/L concentration. Aniline was used as a reference substance for the study.The initial concentration of test chemical was 100 mg/L. Biodegradation was analyzed by using three parameters namely O2 consumption (BOD), TOC removal and test material analysis by HPLC. After 14 days of incubation, percent biodegradation of test chemical was determined to be 72 % by O2 consumption (BOD) parameter 100 % by TOC removal and test material analysis by GC parameter, respectively. Therefore, it is concluded that test chemical is considered to be readily biodegradable in water.
On the basis of above results for test chemical, it can be concluded that the test chemical can be expected to be readily biodegradable in nature.
Biodegradation in water and sediment
Estimation Programs Interface (2018) prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 29.3% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 8.66 days (208 hrs). The half-life (8.66 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of test chemical in sediment is estimated to be 77.916 days (1870 hrs). Based on this half-life value, it indicates that test chemical is not persistent in sediment.
Biodegradation in soil
The half-life period of test chemical in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (2018). If released into the environment, 70.7% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil is estimated to be 17.33 days (416 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.
On the basis of available information, the test chemical can be considered to be readily biodegradable in nature.
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