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Reaction mass ofDisodium [1-{[2-(hydroxy-kO)-3,5-dinitrophenyl]diazenyl-kN1}naphthalen-2-olato(2-)-kO][3-(hydroxy-kO)-4-{[2-(hydroxy-kO)naphthalen-1-yl]diazenyl-kN1}-7-nitronaphthalene-1-sulfonato(3-)]chromate(2-) andDisodium [1-{[2-(hydroxy-kO)-3,5-dinitrophenyl]diazenyl-kN2}naphthalen-2-olato(2-)-kO][3-(hydroxy-kO)-4-{[2-(hydroxy-kO)naphthalen-1-yl]diazenyl-kN1}-7-nitronaphthalene-1-sulfonato(3-)]chromate(2-)
EC number: 944-038-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental starting date - 11 May 1998; Experiment and study completion date - 15 May, 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Code number: FAT 20037/D
- Source and batch No.of test material: EN 101495.CB
- Expiration date of the batch: March, 2003
OTHER SPECIFICS:
- Purity: ca. 79 %,
- Appearance: black, powder
- Solubility: 40 g/L
- Storage: room temperature - Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- - Preparation of stock solution: 1000 mg test substance was dissolved in 200 mL dechlorinated tap water, was homogenized for 10 minutes by ultrasonic treatment and then added up to 1.0 litre. For the preparation of the test concentrations the stock solution was mixed.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra-fish
- Source: River Aquatics, Someren, Netherlands
ACCLIMATION
- Acclimation period: The quality of the fish was checked with an acclimatization time of 61 days in dechlorinated tap water.
- Feeding during the testing: The fish were not fed during the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 142 mg/L CaCO3
- pH:
- 7.9-8.4
- Dissolved oxygen:
- 99 %
- Nominal and measured concentrations:
- Nominal test concentrations: 0, 0.08, 0.18, 0.40, 0.88, 1.9 and 4.3 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beaker
- Material, size, headspace, fill volume: 5 litre glass aquaria with a test volume of 3 litre
- Aeration: Slow aeration
- No. of organisms per vessel: 7
EFFECT PARAMETERS MEASURED: Mortality and behaviour of fish were observed and recorded at 24, 48, 72 and 96 hours - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0.41 - 1.07 mg/L
- Details on results:
- Swimming behaviour was observed at concentrationns above 0.4 mg/L.
- Sublethal observations / clinical signs:
In the test concentrations up to 0.40 mg/L no alteration of the test substance in the test media was observed during the test. In the concentrations of 0.88 up to 4.3 mg/L a small sediment at the bottom of the test vessels was observed after 24 hours. In the control and in the test concentrations up to 0.18 mg/L no mortalities and no abnormal behaviour of the test fish was observed over the test period. Therefore, the NOEC and the LC0 value was determined to be 0.18 mg/L. The LC100 value was determined to be 1.9 mg/L nominal.
Mortalities of fish
Nominal Concentration (mg/L) Test duration 24h 48h 72h 96h Blank 0 0 0 0 0.08 0 0 0 0 0.18 0 0 0 0 0.4 1 2 2 2 0.88 2 4 4 4 1.9 7 - - - 4.3 7 - - - - Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 value to fish was determined to be 0.66 mg/L nominal.
- Executive summary:
An acute study was conducted according to OECD test guideline 203 and EU method C.1 to investigate the toxicity potential of the test item to the zebrafish species. FAT 20037/D was tested at nominal concentrations of 0.08, 0.18, 0.40, 0.88, 1.9 and 4.3 mg test substance/L. Additionally a blank control without test substance was also tested. For each concentration, 7 fish were used. The fish were not fed during the test. In the test concentrations up to 0.40 mg/L, no alteration of the test substance in the test media was observed during the test. In the concentrations of 0.88 up to 4.3 mg/L, a small sediment at the bottom of the test vessels was observed after 24 hours. No mortality was observed in control, 0.08 and 0.18 mg/L concentration. 2/7 and 4/7, fish were found dead at 0.4 and 0.88 mg/L respectively. No fish survived at 1.9 and 4.3 mg/L concentration. Based on the findings of the study, and using the Probit model method, the LC50 value to fish was determined to be 0.66 mg/L nominal.
Reference
Description of key information
The LC50 value for the test substance ranges from 0.66 to 2.3 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.66 mg/L
Additional information
Three studies are available for evaluation of toxicity potential of the test substance to fish.
The key acute study was conducted according to OECD test guideline 203 and EU method C.1 to investigate the toxicity potential of the test item to the zebrafish species. FAT 20037/D was tested at nominal concentrations of 0.08, 0.18, 0.40, 0.88, 1.9 and 4.3 mg test substance/L. Additionally a blank control without test substance was also tested. For each concentration, 7 fish were used. The fish were not fed during the test. In the test concentrations up to 0.40 mg/L, no alteration of the test substance in the test media was observed during the test. In the concentrations of 0.88 up to 4.3 mg/L, a small sediment at the bottom of the test vessels was observed after 24 hours. No mortality was observed in control, 0.08 and 0.18 mg/L concentration. 2/7 and 4/7, fish were found dead at 0.4 and 0.88 mg/L respectively. No fish survived at 1.9 and 4.3 mg/L concentration. Based on the findings of the study, and using the Probit model method, the LC50 value to fish was determined to be 0.66 mg/L nominal.
In a supporting acute fish toxicity study, three groups containing 8 fish each, were exposed to test item concentrations of 0.10, 1.0 and 3.0 mg/L. Observations of mortality were made at 24 and 48 hours during the exposure. No mortality was observed at the concentrations of 0.1 and 1.0 mg/L. However, 7 out of 8 fish died at the concentration of 3.0 mg/L. Hence, the LC50 was calculated to be 1.85 mg/L.
In another supporting acute fish toxicity study conducted on FAT 20037/A, four groups containing 8 fish each, were exposed to test item concentrations of 0.20, 2.0, 20.0 and 100.0 mg/L. Observations of mortality were made at 24 and 48 hours during the exposure. No mortality was observed at the concentration of 0.2 mg/L. At the test concentration of 2.0 mg/L, one fish was found dead at the 48 hours observation. However, 8 out of 8 fish died at the concentrations of 20.0 and 100.0 mg/L. Hence, the LC50 was calculated to be 2.3 mg/L.
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