Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Reactive Brown DYHY 0331/0334 was found to cause no toxic effects to Zebrafish after 96 hours when tested at the limit concentration of nominal 100 mg/L. The LC50 after 96 hours (nominal concentration) was > 100 mg/L
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item Reactive Brown DYHY 0331/0334 to fish (zebrafish) was determined according to the principles of the OECD-Guideline for Testing of Chemicals No. 203.
A semi-static test with daily renewal of the test media was conducted with the nominal limit concentration of 100 mg/L of the test item. Duration of the test was 96 hours. 7 test organisms were exposed to the limit concentration and the control. Water quality parameters temperature, pH-value and oxygen-saturation measured at 0, 24, 48, 72 and 96 hours were within the acceptable limits.
The concentrations of Reactive Brown DYHY 0331/0334 were determined via HPLC-DAD from freshly prepared media after 0 and 72 h and from the corresponding 24 h old media after 24 and 96 h. The measured concentrations of Reactive Brown DYHY 0331 / 0334 were in the range of 100 to 104 % of the nominal value.
All effect values were based on the nominal limit concentration of the test item Reactive Brown DYHY 0331/0334.The results are summarized in the following table:
LC-Values with 95 % Confidence Intervals (0 – 96 hours) of the Test Item
Based on the nominal limit concentration of the test item [mg/L]
Test duration [h] |
LC50 |
24 |
> 100 |
48 |
> 100 |
72 |
> 100 |
96 |
> 100 |
LC100 |
> 100 |
LC0 |
100 |
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