BRUGGOLITE® FF6

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 1997 - November 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: no melting point/range (Decomposition at temperatures > 200 °C)
- Boiling point: no boiling point (decomposition before boiling)
- Vapour pressure: <1 * 10-12 hPa at 25°C (calculation)
- Water solubility: 125.76 g/L at 20°C
- log Pow: <-2
- pKa: = 7,00+/-0,04

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 600 mg/L, 1250 mg/L, 2500 mg/L, 5000 mg/L, 10000mg/L

Vehicle:

yes

Details on test solutions:

The test article is a well soluble powder with strongly reducing properties. A stock solution was made up with 20 L aerated tap-water (c:10g/L). This solution was aerated until the oxygen saturation was >=80%. Prior to the test this solution was diluted with aerated tap-water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Brachydanio rerio: 7 animals/concentration (10 L per vessel)
water: de-chlorinated tap water
supplier: Aquarium Stahl, Rangendingen
Pre-treatment: none (the animaly were held in the laboratory for at leas 12 days before testing; mortality: 0%)
light: 14 h light, 10 h dark , illumination: 60-Watt Gro-Lux
areation: permanent with pasteur pipettes
feeding: no feeding during the test
size: 3.1-3.5 cm
weight: 0.27 - 0.39 g
corpulence factor 0.9 -0.91 g/cm³ [G(g) x 100 /L(cm³)]

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

96 h

Hardness:

Carbonate hardness: 8 mg CaCO3/L
total hardness: 8.6 °dH

Test temperature:

23°C +/- 2°C

pH:

7.9 - 8.4

Dissolved oxygen:

> 80% in all vessels at various observation times (0h, 24h, 48h, 72h, 96h)

Salinity:

de-chlorinated tap-water;
Nitrate: 3 mg/L;
Nitrite: <0.1 mg/L;
Zn: 0.8 mg/L;
Fe/Cd/Pb/Cu/Ni:<0.1 mg/L

Nominal and measured concentrations:

see table 1

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 L solid-glass fish tanks
- Aeration: Pasteur pipettes, pump
- No. of animals per concentration: 7

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap-water
- Metals:Zn: 0.8 mg/L; Fe/Cd/Pb/Cu/Ni:<0.1 mg/L
- Chlorine: dechlorined water

Reference substance (positive control):

yes

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 7 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 7 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 7 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 5 000 mg/L

Details on results:

OBSERVATIONS:
-swarm behaviour
-activity
-surfacing
-balance
-mortalities
-gills (breathing-frequency)

-600 mg/L: After 96 h the animals behave as in the control
-2500 mg/l: analogue 600 mg/L
-5000 mg/l: analogue 600 mg/L
-10000 mg/l: after 72 h one animal died. After 96 h the surviving animals behave as in the control

Results with reference substance (positive control):

- Mortality: 0
see table 2

Sublethal observations / clinical signs:

table 2: Cumulative mortality [%] at various observation times

time [h]	control	600 [mg/L]	1250 [mg/L]	2500 [mg/l]	5000 [mg/L]	10000 [mg
24	0	0	0	0	0	0
48	0	0	0	0	0	0
72	0	0	0	0	0	14 .3
96	0	0	0	0	0	14.3

Validity criteria fulfilled:

yes

Conclusions:

The test substance is not acute toxic to fish.
LC50 (96h) >7400 mg/l

Description of key information

Not acute toxic to fish.
LC50 (96h) >7400 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

7 400 mg/L