3-[[3-(dimethylamino)propyl]amino]propiononitrile

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-[[3-(dimethylamino)propyl]amino]propiononitrile

EC Number:

274-159-1

EC Name:

3-[[3-(dimethylamino)propyl]amino]propiononitrile

Cas Number:

69852-45-5

Molecular formula:

C8H17N3

IUPAC Name:

3-{[3-(dimethylamino)propyl]amino}propanenitrile

impurity 1

Reference substance name:

3,3'-[[3-(dimethylamino)propyl]imino]bispropiononitrile

EC Number:

275-331-9

EC Name:

3,3'-[[3-(dimethylamino)propyl]imino]bispropiononitrile

Cas Number:

71326-27-7

Molecular formula:

C11H20N4

IUPAC Name:

3,3'-{[3-(dimethylamino)propyl]imino}dipropanenitrile

Test material form:

liquid

Remarks:

colorless

Test animals

Species:

rabbit

Strain:

Himalayan

Details on test animals or test system and environmental conditions:

The test was performed on 3 male and 3 female rabbits of the Himalayan breed weighing 1.5 to 2 kg. They were housed individually in metal cages, were kept at a constant room temperature of 22 + 1° C, at a relative humidity of 55 + 5 % and on a 10 hours light cycle day. The animals received ad libitum Standard rabbit food - NAFAG, Gossau SG - and water. Prior to treatment,they were adapted to our laboratories for a minimum of 4 days.

Test system

Type of coverage:

occlusive

Preparation of test site:

other: Shaved. Left side slightly scarified.

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

0.5ml of test material was applied

Duration of treatment / exposure:

Dressings were removed after after 24 hours exposure

Observation period:

Observations were made 24 and 72 hours after application of test material

Number of animals:

6 animals, 3 male and 3 female

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Remarks:

Intact skin

Time point:

24 h

Score:

2.3

Max. score:

4

Reversibility:

not fully reversible within: 72 h

Remarks on result:

positive indication of irritation

Results 2

Irritation parameter:

erythema score

Basis:

mean

Remarks:

Scarified skin

Time point:

24 h

Score:

4

Max. score:

4

Reversibility:

not fully reversible within: 72 h

Remarks on result:

positive indication of irritation

Results 3

Irritation parameter:

erythema score

Basis:

mean

Remarks:

Intact skin

Time point:

72 h

Score:

3

Max. score:

4

Reversibility:

not fully reversible within: 72 h

Remarks on result:

positive indication of irritation

Results 4

Irritation parameter:

erythema score

Basis:

mean

Remarks:

Scarified skin

Time point:

72 h

Score:

3.8

Max. score:

4

Reversibility:

not fully reversible within: 72 h

Remarks on result:

positive indication of irritation

Results 5

Irritation parameter:

edema score

Basis:

mean

Remarks:

Intact skin

Time point:

24 h

Score:

2.2

Max. score:

4

Reversibility:

not fully reversible within: 72 h

Remarks on result:

positive indication of irritation

Results 6

Irritation parameter:

edema score

Basis:

mean

Remarks:

Scarified skin

Time point:

24 h

Score:

2.2

Max. score:

4

Reversibility:

not fully reversible within: 72 h

Remarks on result:

positive indication of irritation

Results 7

Irritation parameter:

edema score

Basis:

mean

Remarks:

Intact skin

Time point:

72 h

Score:

0.5

Max. score:

4

Reversibility:

not fully reversible within: 72 h

Remarks on result:

positive indication of irritation

Results 8

Irritation parameter:

edema score

Basis:

mean

Remarks:

Scarified skin

Time point:

72 h

Score:

2.8

Max. score:

4

Reversibility:

not fully reversible within: 72 h

Remarks on result:

positive indication of irritation

Results 9

Irritation parameter:

erythema score

Basis:

mean

Remarks:

intact skin

Time point:

24/48/72 h

Score:

2.7

Max. score:

4

Reversibility:

not fully reversible within: 72h

Remarks on result:

positive indication of irritation

Results 10

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

1.1

Max. score:

4

Reversibility:

not fully reversible within: 72h

Remarks on result:

positive indication of irritation

Any other information on results incl. tables

Animal No. Sex	Skin Reaction	24 hours after application		72 hours after application
		Intact skin	Scarified skin	Intact skin	Scarified skin
1 ♂	Erythema	2	4	2	4
	Edema	2	2	0	1
2 ♂	Erythema	2	4	2	3
	Edema	2	2	0	13
3 ♂	Erythema	2	4	3	4
	Edema	2	2	0	0
4 ♀	Erythema	2	4	4	4
	Edema	3	3	1	1
5 ♀	Erythema	4	4	4	4
	Edema	2	2	1	1
6 ♀	Erythema	2	4	3	4
	Edema	2	2	1	1

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

The primary irritation index, a measure of the acute irritation to the rabbit skin was 4.7. This indicates that the test material is a moderate irritant to the rabbit skin.