1-butyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Principles of method if other than guideline:

- according to H.F. Smyth and C.P. Carpenter, J. Ind. Hyg. Tox. 26, 269 (1944).
- principle of the inhalation hazard test (rat):
This test (IRT) was performed in principle as described in OECD Guideline 403. It demonstrates the toxicity of an atmosphere saturated with vapors of the volatile com-ponents of a test substance at the temperature chosen for vapor generation (usually 20°C). Young adult laboratory rats were purchased from a breeder. In general, the source and strain of the animals were not documented. Several groups of usually 3 rats per sex were exposed sequentially to the vapors, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for different time periods (e.g. 3 min, 10 min, 1, 3 or 7 or 8 hours). The exposure time not causing lethality was usually tested twice. No analytical determination of the atmosphere concentrations was performed. The nominal concentration usually can be calculated as quotient of the amount of test substance weight loss during the exposure, which is given in the raw data, and the amount of air used during the exposure. Group-wise documentation of clinical signs was performed over the 7- to 14- day study period. Body weight of groups was determined before the start of the study and at the end of the observation period in surviving animals. The clinical signs and findings were reported in summarized form. More details can usually be inferred as mentioned for the acute oral studies.
The study allows for an estimate of the length of time required to cause severe toxic effects resulting from exposure to an atmosphere saturated with volatile components of the test substance. The exposure time causing 50% lethality (LT50) can be estimated from such a study as described for the LD50. Furthermore, using the nominal concentration, vapor pressure and LT50, in many cases a 4-hour LC50 can be estimated using Haber’s law.

GLP compliance:

no

Test type:

other: inhalation hazard test

Test material

Test material information

Constituent 1

Reference substance name:

1-butyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile

EC Number:

254-296-3

EC Name:

1-butyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile

Cas Number:

39108-47-9

Molecular formula:

C11H14N2O2

IUPAC Name:

1-butyl-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile

Details on test material:

Name of the test substance used in the study report: N-Butyl-2-hydroxy-3-cyan-4-methylpyridon(6)

Test animals

Species:

rat

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: dust

Duration of exposure:

8 h

Concentrations:

mean concentration: 18.63 mg/l

No. of animals per sex per dose:

12

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

other: mortality

Effect level:

other: no mortality after 8h exposure

Based on:

test mat.

Exp. duration:

8 h

Mortality:

none

Clinical signs:

other: none

Gross pathology:

nothing abnormal detected