Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
yes (incl. QA statement)
Test type:
acute toxic class method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1',2'-dihydro-6'-hydroxy-3,4'-dimethyl-2'-oxo-1,3'-bipyridinium chloride
EC Number:
279-301-6
EC Name:
1',2'-dihydro-6'-hydroxy-3,4'-dimethyl-2'-oxo-1,3'-bipyridinium chloride
Cas Number:
79817-70-2
Molecular formula:
C12H13N2O2.Cl
IUPAC Name:
6'-hydroxy-3,4'-dimethyl-2'-oxo-1',2'-dihydro-1,3'-bipyridinium dichloride
Test material form:
liquid
Details on test material:
Active su
Specific details on test material used for the study:
The test material was supplied as dark brown liquid, stored at room temperature.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
Male and female Sprague-Dawley strain rats weighed 147-176 g, and 140-152 g respectively and were approximately five to eight weeks old. They were separated by sex. Room temperature 19-22ºC and humidity of 44-58%

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Details on oral exposure:
All animals were dosed once only by gavage using a metal cannula attached to a graduate syringe.
Doses:
The volume administrated to each animal was calculated according to its fasted bodyweight at the time of dosing.
No. of animals per sex per dose:
5 male, 5 female
Control animals:
yes
Details on study design:
The animals were observed for death or overt signs of toxicity 0.5, 1, 2 and 4 hours after dosing and subsequently once daily for 14 days

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Mortality:
There were no deaths.
Clinical signs:
other: No signs of systemic toxicity were noted during the study.
Gross pathology:
No abnormalities were noted at necropsy.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The acure oral median lethal dose (LD50) of the test materials, HMPPJC, in the Sprague-Dawley strain rat was found to be greater than 2000 mg/kg bodyweight. No symbol and risk phrase are required according to EEC labelling regulations.