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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-03-21 to 1991-04-23
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Monitored GLP study followed international guidelines

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-chloro-2,5-dimethoxyaniline
EC Number:
228-782-0
EC Name:
4-chloro-2,5-dimethoxyaniline
Cas Number:
6358-64-1
Molecular formula:
C8H10ClNO2
IUPAC Name:
4-chloro-2,5-dimethoxyaniline
Constituent 2
Reference substance name:
4-chloro-2,5-dimethoxyanilin
IUPAC Name:
4-chloro-2,5-dimethoxyanilin

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Reference substance (positive control):
yes
Remarks:
Cu(II)SO4*5H2O

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
75 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
260 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
900 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Any other information on results incl. tables

Reference substance (copper sulfate)

@ 100 mg/L respiration inhition of 45%

Validity criteria: 40 - 60%

valid: yes

Test substance

see block effect contentrations

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance was tested for its toxicity to microorganisms stp.
The EC50 is well above 100 mg/L.
Executive summary:

The test substance was tested in a valid GLP monitored OECD 209 study. The results indicate a NOEC <75 mg/L, an EC50 of 260 mg/L and an EC80 of 900 mg/L.