Sodium phenoxyacetate hemihydrate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

other: Validation of the analytical method.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

2008

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Version / remarks:

1996

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch No.: 31233510

Analytical monitoring:

yes

Details on sampling:

For determination of the test item concentrations, five replicate samples were taken from the test solution (100 mg/L) and three replicate samples were taken from the control group at the start and at the end of the test.

Vehicle:

yes

Details on test solutions:

The test solution used in the test was prepared by mechanical dispersion. An amount of 0.5 g test item was dissolved in 5000 mL dilution water (ISO medium) in order to obtain the nominal concentration of 100 mg/L. The test solution was freshly prepared in the testing laboratory just before introduction of the test animals (start of the experiment).
Reconstituted water (ISO medium) was prepared according to Annex 2 of the OECD guideline 203.

Test organisms (species):

other: Brachydanio rerio

Details on test organisms:

Species: Zebrafish (Brachydanio rerio)
Origin: Akvárium magazin Kft. (Pasaréti Gyula) 1222 Budapest, Dévény u. 36.
Number of animals: There were 10 animals at the treated and control group respectively (no parallels were used).
Age and size of animals: Juveniles; size within a range of 2.0 ± 1 cm. The loading of the test aquaria did not exceed 1.0 g / L.
Sex: Both female and male (were not separated).
Animal health: Apparently healthy animals were used in the test.
Acclimatization, holding of fish prior to the study: Fish were held for at least 12 days before test initiation in the fish laboratory of TOXI-COOP ZRT. under the same conditions as used during the exposure period.
During holding, fish were fed with appropriate, commercial diet for fish at least three times per week until one day before the test start. The health of the breeding was continuously monitored and any mortality or abnormal behaviour recorded. No mortality occurred in seven days before the start of the experiment, therefore the batch was considered to be acceptable for testing.
Light: 12 to 16 hours photoperiod daily.
Temperature: 21 – 25°C, constant within ± 1°C.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

No.

Hardness:

Hardness: approximate theoretical total hardness of 249 mg/L (as CaCO3).

Test temperature:

21.9 - 22.1 °C

pH:

The measured pH values were in the range of 7.11 – 7.97 during the test.

Dissolved oxygen:

74.3 - 85.7 %

Salinity:

/

Conductivity:

/

Nominal and measured concentrations:

Nominal: 0 and 100 mg/L.
Actual: 0 for the control. The average measured concentration of Sodium Phenoxyacetate was 99.2 mg/L at the start and 99.0 mg/L at the end of the test.

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
Reconstituted water (ISO medium), prepared according to Annex 2 of the OECD guideline 203, was used.

OTHER TEST CONDITIONS
The test was carried out without adjustment of pH.
The light-dark cycle during the test was 16 hours light (artificial illumination) and 8 hours darkness.
The loading of the test aquaria did not exceed 1.0 g / L

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: sub-lethal effects

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: sub-lethal effects

Details on results:

There were no mortality and any sub-lethal effect observed in 10 fish in the control group or in 10 fish exposed to a test concentration of 100 mg/L.

Results with reference substance (positive control):

/

Reported statistics and error estimates:

Not relevant.

Validity criteria fulfilled:

yes

Conclusions:

In this 96-hour static acute toxicity test on Zebrafish (Brachydanio rerio) the test item Sodium Phenoxyacetate had no toxic effect on fish up to a concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be >100 mg/L. The 96-h NOEC was determined to be 100 mg/L.

Executive summary:

A 96-hour static acute toxicity test on Zebrafish (Brachydanio rerio) was performed according to the OECD guideline 203. The test item Sodium Phenoxyacetate had no toxic effect on fish up to a concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be >100 mg/L. The 96-h NOEC was determined to be 100 mg/L.

Description of key information

The LC50 of the test substance to Zebrafish is >100 mg/L.

The LC50 is not 100 mg/L, as one is forced to enter in the field below, but the LC50 > 100 mg/kg.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L