Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 Oct - 02 Nov 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test: Determination of toxic effects of the test item to anaerobic bacteria through reduction of gas production compared to untreated control
- Short description of test conditions:
- test item: Remazol-Brillantgrün 6B
- test duration: 7 days
- temperature 37°C +/- 1°C
- concentrations of the test item: 700 mg/L to 1000 mg/L with ech 3 replicates
- reference item: Dodecyldimethylbenzylammoniumchlorid 50, 100, 200 mg/L
- test vessels: 100 mL bottles filled with 30 mL test medium
- Parameters analysed / observed: injection of phosphoric acid (85%) 30 minutes before measuring the CO2 pressure in the headspace of the bottles..

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts

EC Number:

305-644-9

EC Name:

Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts

Cas Number:

94891-43-7

Molecular formula:

C32 H16 N8 Ni . C8 H11 N O6 S2 . 2 Na

IUPAC Name:

Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution: 1000 mg test item to 450 mL, pH adjusted from 4,7 to 7,2, stirred for 1,5 hours at 22°C, afterwards pH adjusted from 6,9 to 7,3, stock solution finally filled to 500 mL (concentration: 2000 mg/L). clear solution without undissolved test item
- Controls: yes: test vessel with inoculum, without test item
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): -
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no undissolved material

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: no
- Name and location of sewage treatment plant where inoculum was collected: anerobic activated sludge from domestic waste water treatment plant Kriftel
- Preparation of inoculum for exposure: after pretreatment, addition of nutrition solution according to ISO 11734
- Pretreatment: 9 days at 35°C without additing of nutrient solution
- Initial biomass concentration: concentration of activated sludge in test vessels: 3,5 g/L (dry weight)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

9 d

Test conditions

Test temperature:

37°C +/- 1°C

Nominal and measured concentrations:

nominal concentrations 700 mg/L and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL bottles
- Material, size, headspace, fill volume: fill volume: 30 mL
- No. of vessels per concentration (replicates): 3
- Sludge concentration (weight of dry solids per volume): 3.5 g/L (dry weight)

Reference substance (positive control):

yes

Remarks:

Dodecyldimethylbenzyl ammonium chloride

Results and discussion

Effect concentrations

Key result

Duration:

7 d

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of anaerobic gas production

Any other information on results incl. tables

concentration [mg/L]	inhibition [%]
1000	10
700	6

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The 7-d EC20 to anaerobic bacteria was determined >1000 mg/L (nominal) based on anaerobic gas production.