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Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18 Oct - 02 Nov 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
- Principle of test: Determination of toxic effects of the test item to anaerobic bacteria through reduction of gas production compared to untreated control
- Short description of test conditions:
- test item: Remazol-Brillantgrün 6B
- test duration: 7 days
- temperature 37°C +/- 1°C
- concentrations of the test item: 700 mg/L to 1000 mg/L with ech 3 replicates
- reference item: Dodecyldimethylbenzylammoniumchlorid 50, 100, 200 mg/L
- test vessels: 100 mL bottles filled with 30 mL test medium
- Parameters analysed / observed: injection of phosphoric acid (85%) 30 minutes before measuring the CO2 pressure in the headspace of the bottles..
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts
EC Number:
305-644-9
EC Name:
Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts
Cas Number:
94891-43-7
Molecular formula:
C32 H16 N8 Ni . C8 H11 N O6 S2 . 2 Na
IUPAC Name:
Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution: 1000 mg test item to 450 mL, pH adjusted from 4,7 to 7,2, stirred for 1,5 hours at 22°C, afterwards pH adjusted from 6,9 to 7,3, stock solution finally filled to 500 mL (concentration: 2000 mg/L). clear solution without undissolved test item
- Controls: yes: test vessel with inoculum, without test item
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): -
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no undissolved material

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Laboratory culture: no
- Name and location of sewage treatment plant where inoculum was collected: anerobic activated sludge from domestic waste water treatment plant Kriftel
- Preparation of inoculum for exposure: after pretreatment, addition of nutrition solution according to ISO 11734
- Pretreatment: 9 days at 35°C without additing of nutrient solution
- Initial biomass concentration: concentration of activated sludge in test vessels: 3,5 g/L (dry weight)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
9 d

Test conditions

Test temperature:
37°C +/- 1°C
Nominal and measured concentrations:
nominal concentrations 700 mg/L and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL bottles
- Material, size, headspace, fill volume: fill volume: 30 mL
- No. of vessels per concentration (replicates): 3
- Sludge concentration (weight of dry solids per volume): 3.5 g/L (dry weight)
Reference substance (positive control):
yes
Remarks:
Dodecyldimethylbenzyl ammonium chloride

Results and discussion

Effect concentrations
Key result
Duration:
7 d
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of anaerobic gas production

Any other information on results incl. tables

 concentration [mg/L]  inhibition [%]
 1000  10
 700  6

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The 7-d EC20 to anaerobic bacteria was determined >1000 mg/L (nominal) based on anaerobic gas production.

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