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EC number: 283-810-9 | CAS number: 84713-17-7
Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water: sewage treatment simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-04-30 to 1991-06-07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)
- Version / remarks:
- (1981)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.10 (Biodegradation: Activated Sludge Simulation Test)
- Version / remarks:
- (1988)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2,4(or 2,4,4)-trimethylhexanedinitrile
- EC Number:
- 283-810-9
- EC Name:
- 2,2,4(or 2,4,4)-trimethylhexanedinitrile
- Cas Number:
- 84713-17-7
- Molecular formula:
- C9H14N2
- IUPAC Name:
- 2,2,4-trimethylhexanedinitrile; 2,4,4-trimethylhexanedinitrile
- Details on test material:
- 2,2,4(or 2,4,4)-Trimethylhexanedinitrile of Hüls AG, Batch No. 0802911200, ID No. 3630/81302, purity 96.3%
Constituent 1
- Radiolabelling:
- no
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on source and properties of surface water:
- synthetic sewage was used (based on tap water)
- Details on source and properties of sediment:
- no sediment was used for the study
- Details on inoculum:
- INOCULUM
- Source: municipal WWTP Marl-West, sampled 30 Apr 1991
- Pretreatment: fed into test apparatus approx. 40 min after sampling - Duration of test (contact time):
- 3 h
Initial test substance concentrationopen allclose all
- Initial conc.:
- 10 mg/L
- Based on:
- DOC
- Initial conc.:
- 24 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- METHOD OF PREPARATION OF TEST SOLUTION:
- Stock solution made of 50 g test substance and 5 l deionized water 10 g test substance/l = 4175 mg DOC/l
DURATION OF THE TEST: 38 days
TEST SYSTEM
- Culturing apparatus: flow-through
- Number of culture flasks per concentration: 1
- Aeration device: pump
- Measuring equipment: TOC 500 infrared analyzer
- Closed vessels used: no
- Test temperature: 19.6 - 22.2 °C
- Flow rate: 1 l/hour; mean retention time: 3 hours.
- Aeration: Sufficient to keep oxygen >= 2 mg/l
CONTROLS: synthetic waste water; exchange of sludge (1.5 l) once daily
SAMPLING
- DOC determination daily (except weekends) after mixing of 24 hour collected effluent;
- Activated sludge dry weight content determined gravimetrically once per week
Results and discussion
Mean total recovery
- Compartment:
- other: water / sediment, material (mass) balance
- Remarks on result:
- other: no data available
% Degradation
- % Degr.:
- 7.43
- St. dev.:
- 4.3
- Parameter:
- DOC removal
- Sampling time:
- 3 h
- Remarks on result:
- other: mean value
- Transformation products:
- not measured
- Details on transformation products:
- Transformation products were not measured
- Evaporation of parent compound:
- not measured
- Volatile metabolites:
- not measured
- Residues:
- not measured
Any other information on results incl. tables
21 of the 24 measured values were considered for calculation of degradation of the test substance.
Applicant's summary and conclusion
Validity criteria
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The mean degree of elimination of 2,2,4(or 2,4,4)-trimethylhexanedinitrile at the plateau phase was 7.43% (CL: 3.14-11.71%)
- Executive summary:
The ultimate biodegradation of 2,2,4(or 2,4,4)-trimethylhexanedinitrile was determined by aerobic micro-organisms in a continuously operated test system simulating the activated sludge process according to OECD TG 303 A. The DOC was determinded in frequently taken samples of the effluents over a period of 36 days. The mean degree of elimination of test substance at the plateau phase was 7.43% (CL: 3.14-11.71%) indicating that only less degradation took place.
The study was assessed as "reliable without restrictions".
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