1,1-dimethoxy-2-phenylethane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 - 27 Jun 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

TOC determination

Details on sampling:

- Concentrations: all test item concentrations and the control
- Sampling method: at test start and test end

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test item was directly weighed into test water and treated for 60 sec at 8000 rpm with an ultra-turrax and stirred afterwards for 24 h with an a magnetic stirrer. Undissolved particies were removed via a folded filter of the pore size 7-12 µm.
- Controls: yes, test medium control
- Eluate: no
- Differential loading: yes

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age of test animals: 0 - 24 h
- Keeping conditions: according to internal SOP

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Separation of test animals according to size before start of test: 1st sieve: 14.06.1999 at 2.00 p.m. and 2nd sieve: 15.06.1999 at 8:00 a.m.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

15.5 °dH (M4 medium)

Test temperature:

19.4 °C (at test end, control and test item concentration)

pH:

8.1 (at test end, control)
8.0 (at test end, test item concentration)

Dissolved oxygen:

8.6 mg/L (at test start and test end, control and test item concentration)

Nominal and measured concentrations:

nominal: control, 100 mg/L

Details on test conditions:

TEST SYSTEM
Test vessel:
- Type: open
- Material, size, headspace, fill volume: cylindrical test vessels, diameter 4.0 cm, height 6.5 cm, 20 mL test medium
- Aeration: not ventilated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-Medium according to Elendt and BGA (1992)

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark

EFFECT PARAMETERS MEASURED: Immobilisation was measured after 24 and 48 h.

VEHICLE CONTROL PERFORMED: no (not required)

RANGE-FINDING STUDY: no range-finding test was performed

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 97.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: arithmetic mean of analytical values

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 97.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: arithmetic mean of analytical values

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no

Analytical Results:

All values refer to TOC determinations. According to the relevant product informations 1 mg/L TOC equals to 1.4 mg/L of the test substance (structural formula: C10-H14-02; molecular weight: 166.24 g/mole).

The test result refers to a concentration of the test substance, which was calculated directly from analytically determined TOC values. According to the relevant product informations, 1 mg/L equals to 1.4 mg/L of the test substance structural formula; molecular weight: 166.24 g/mole).

Nominal concentration test substance	Actual concentrations (based on arithmetic mean measured concentrations)	TOC results by start of test and after 48 hours [mg/L]
[mg/L]	[mg/L]	0 hours	48 hours
Control	0	< 2	3
100	97.3	71	68

Validity criteria fulfilled:

yes

Conclusions:

The test item had no acute toxic effects on Daphnia magna in a 48-h static test according to EU Method C.2 and GLP up to 100 mg/L (nominal). Immobilisation of Daphnia magna was observed after 48 h resulting in an EC50 > 100 mg/L (> 97.3 mg/L based on the arithmetic mean).

Description of key information

EC50 (48 h) >100 mg/L (EU C.2, Daphnia magna, nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

One experimental study is available investigating the short-term effects of the substance to aquatic invertebrates. The study was performed according to the EU Method C.2 (GLP) with the water flea Daphnia magna. A limit test with a nominal concentration of 100 mg/L including a control was performed under static conditions. Test concentrations were verified by TOC-analysis at the beginning and end of the exposure period. The nominal concentration of the highest test item concentration was 71 mg/L at test start (corresponds to 99.4 mg/L based on arithemtic mean measured concentration) and 68 mg/L at test end (corresponds to 95.2 mg/L based on arithemtic mean measured concentration). Immobilisation of D.magna was not observed after 48 h resulting in an EC50 (48 h) of >100 mg/L (>97.3 mg/L based on the arithmetic mean measured concentration).