Cyclododecane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Cited as Directive 92/69/EEC, C.1

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cyclododecane

EC Number:

206-033-9

EC Name:

Cyclododecane

Cas Number:

294-62-2

Molecular formula:

C12H24

IUPAC Name:

cyclododecane

Details on test material:

- cyclododecane of Hüls AG, produced 30 Aug. 1996, purity 99.8% (GC-FID area);
- white, crystalline
- ID No. 0637/81794, PR No. 1613/960924

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- 1 g test substance was stirred for ca. 18 hours in  water and filtered (Water Accomodated Fraction). Test solutions were prepared daily.

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISMS
- Supplier: Bio International B.V., Someren (NL)
- Age/size/weight/loading: length 3-4 cm, weight ca. 0.79 g
- Feeding: TRUOVIT, ca. 1 % of body weight daily
- Pretreatment: 14 days quarantine
- Feeding during test: no

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

ca. 13 degree (German hardness)

Test temperature:

19.6-22.1 degree C (mean: 21)

pH:

- pH: 8.0-8.6

Dissolved oxygen:

93-100 % of saturation

Details on test conditions:

DILUTION WATER
- Source: drinking water (Gelsenwasser AG)
- Aeration: continuously during test

TEST SYSTEM
- Concentrations: 1.0 mg/l (maximum soluble)
- Renewal of test solution: daily
- Exposure vessel type: ca. 20 l aquaria with 10 l test solution
- Number of replicates, fish per replicate: 1 replicate with 10 fish
- Adjustment of pH: no - Photoperiod: 16 h/day

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The arithmetical mean TOC concentration was 0.89 mg/L corresponding to 1 mg/L test substance

Any other information on results incl. tables

Sublethal observations / clinical signs:

At the highest concentration which could be obtained by Water Accomodated Fraction (WAF) under the conditions of the test, no mortality could be observed.
1/10 control animals died on day 4.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 (96 h) was determined as >1.0 mg/L indicating that the test substance has no acute toxicity to fish up to the limit of the water solubility

Executive summary:

The acute toxicity of cyclododecane on Cyprinus carpio was investigated by Hüls AG (1997) in a 96 -h test according to EU method C.1. The concentration tested (limit test) was obtained by Water Accomodated Fraction. The test item concentration was analysed by means of TOC. The mean concentration was determined as 1.0 mg/L. The following endpoint was derived

LC50 (96 h): >1 mg/L

The study was assessed as "reliable without restrictions".