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EC number: 284-395-7 | CAS number: 84870-65-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
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- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 November 1987 to 25 November 1987
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- OECD Guidelines for Testing of Chemicals (1981) No. 402 "Acute Dermal Toxicity".
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
- EC Number:
- 284-395-7
- EC Name:
- 2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
- Cas Number:
- 84870-65-5
- Molecular formula:
- C19H18N6O2
- IUPAC Name:
- 2-[[4-(diethylamino)-2-methylphenyl]azo]-5-nitrobenzene-1,3-dicarbonitrile
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- No further details specified in the study report.
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Five male and five female Sprague-Dawley CFY strain rats were supplied by Interfauna (UK) Limited, Wyton, Huntingdon, Cambridgeshire. At the start of the study the males weighed 232 - 245g, and the females 241 - 258g, and were approximately ten to fourteen weeks old. After a minimum acclimatisation period of five days the animals were selected at random and given a unique number within the study by ear punching and a number written on a cage card.
The animals were housed in solid-floor polypropylene cages with sawdust bedding and allowed free access to mains drinking water and food (Rat and Mouse Expanded Diet No. 1, Special Diet Services Limited, Witham, Essex, U.K.) throughout the study.
The animal room was maintained at a temperature of 19 – 22 °C and relative humidity of 45 - 65%. The rate of air exchange was approximately 15 changes per hour and the lighting was controlled by a time switch to give 12 hours light and 12 hours darkness.
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- Approximately twenty-four hours prior to the commencement of the test the back and flanks of each animal were clipped free of hair using veterinary clippers to expose a skin area of approximately 6 cm x 12 cm.
The appropriate amount of the test material, as received, pre-weighed into a glass vial, was applied uniformly to an area of shorn skin approximating to 10% of the total body surface area which had previously been moistened with arachis oil B.P. A piece of surgical gauze measuring 7 cm x 4 cm was placed over the treatment area and semi-occluded with a double layer of elastic adhesive bandage (ELASTOPLAST) wrapped around the trunk of the rat. The bandage was tightened sufficiently to prevent the animal wriggling free.
The rats were individually caged for the 24-hour exposure period to prevent mutual interference with the dressing. After the 24-hour contact period the bandage was carefully removed and the treated skin and surrounding hair wiped with moist cotton wool to remove any residual test material. The animals were then caged in groups of five by sex. - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 10 (5 males/5 females)
- Control animals:
- no
- Details on study design:
- All animals were observed for overt signs of toxicity and mortality 1 and 4 hours after dosing and subsequently at least once daily for fourteen days.
Individual bodyweights were recorded on the day of treatment (day 0) and on days seven and fourteen.
All animals were subjected to a gross necropsy examination for any macroscopic abnormalities. No tissues were retained. - Statistics:
- Using the mortality data, an assessment of the acute dermal median lethal dose (LD50) of the test material was made. Clinical observations, bodyweight records and necropsy data were examined for any adverse, but non-lethal effects resulting from treatment.
Results and discussion
- Preliminary study:
- Not specified in the study report.
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality occured.
- Clinical signs:
- other: No signs of systemic toxicity or skin irritation were noted during the study period.
- Gross pathology:
- No abnormalities were noted at necropsy of animals killed at the end of the study period.
- Other findings:
- All application sites were stained blue by the test material orr removal of the dressings and up to five to six days after treatment.
Any other information on results incl. tables
The mortality data are summarised as follows:
DOSE LEVEL mg/kg |
SEX |
DEATHS ON DAY: |
TOTAL DEATHS |
||||||||
0 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8-14 |
|||
2000 |
MALE |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0/10 |
FEMALE |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
INDIVIDUAL CLINICAL OBSERVATIONS
TEST MATERIAL: DISPERSIONSBLAU F-60 768
DOSE LEVEL mg/kg |
ANIMAL NUMBER & SEX |
HOURS AFTER DOSING |
DAYS AFTER DOSING |
||||||||||||||
1 |
4 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
||
2000 |
1-0 MALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1-1 MALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
1-2 MALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
1-3 MALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
1-4 MALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2-0 FEMALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2-1 FEMALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2-2 FEMALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2-3 FEMALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
2-4 FEMALE |
0 |
0 |
0 St |
0 St |
0 St |
0 St |
0 St |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
St = site of application stained blue by test material 0 = no systemic toxicity detected
INDIVIDUAL BODYWEIGHTS AND WEEKLY BODYWEIGHT INCREASES
TEST MATERIAL: DISPERSIONSBLAU F-60 768
DOSE LEVEL mg/kg |
ANIMAL NUMBER & SEX |
BODYWEIGHT (g) AT DAY |
INCREMENT (g) DURING WEEK |
|||
0 |
7 |
14 |
1 |
2 |
||
2000 |
1-0 MALE |
232 |
281 |
345 |
49 |
64 |
1-1 MALE |
243 |
308 |
392 |
65 |
84 |
|
1-2 MALE |
245 |
267 |
362 |
22 |
95 |
|
1-3 MALE |
234 |
280 |
345 |
46 |
65 |
|
1-4 MALE |
237 |
288 |
349 |
51 |
61 |
|
2-0 FEMALE |
250 |
281 |
302 |
31 |
21 |
|
2-1 FEMALE |
241 |
250 |
278 |
9 |
28 |
|
2-2 FEMALE |
252 |
278 |
298 |
26 |
20 |
|
2-3 FEMALE |
244 |
259 |
272 |
15 |
13 |
|
2-4 FEMALE |
258 |
274 |
292 |
16 |
18 |
INDIVIDUAL NECROPSY FINDINGS
TEST MATERIAL: DISPERSIONSBLAU F-60 768
DOSE LEVEL mg/kg |
ANIMAL NUMBER & SEX |
MACROSCOPIC OBSERVATIONS |
|
2000 |
1-0 MALE |
Killed day 14 |
No abnormalities detected |
1-1 MALE |
Killed day 14 |
No abnormalities detected |
|
1-2 MALE |
Killed day 14 |
No abnormalities detected |
|
1-3 MALE |
Killed day 14 |
No abnormalities detected |
|
1-4 MALE |
Killed day 14 |
No abnormalities detected |
|
2-0 FEMALE |
Killed day 14 |
No abnormalities detected |
|
2-1 FEMALE |
Killed day 14 |
No abnormalities detected |
|
2-2 FEMALE |
Killed day 14 |
No abnormalities detected |
|
2-3 FEMALE |
Killed day 14 |
No abnormalities detected |
|
2-4 FEMALE |
Killed day 14 |
No abnormalities detected |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute dermal LD50 of the test material, Dispersionsblau F 60 768, to the rat was found to be greater than 2000 mg/kg bodyweight.
The substance is not classifiable according to CLP criteria. - Executive summary:
A study was performed to assess the acute dermal toxicity of the test material in the Sprague-Dawley CFY strain rat. The method used followed that described in the OECD Guidelines for Testing of Chemicals (1981) No. 402 "Acute Dermal Toxicity".
A group of ten animals (five males and five females), was given a single, 24-hour, semi-occluded dermal application of the test material to intact skin, at a dose level of 2000 mg/kg bodyweight.
There were no deaths. There were no signs of systemic toxicity or skin irritation over the study period. All application sites were stained blue by the test material.
All animals showed expected gains in bodyweight over the study period.
No abnormalities were noted at necropsy of animals killed at the end of the study period.
The acute dermal median lethal dose (LD50) of the test material, DISPERSIONSBLAU F-60 768, to the rat was found to be greater than 2000 mg/kg bodyweight.
The substance is not classified according to CLP criteria.
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