14H-benz[4,5]isoquino[2,1-a]perimidin-14-one

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

A study on the acute inhalation toxicity of Macrolex Rot E2G (CAS no 6829-22-7) on rats has been conducted in accordance with OECD TG#403 (2009). One group of rats was nose-only exposed to the solid aerosol of the test item at the actual concentration of 1817 mg/m³ (maximum attainable concentration). Rats exposed to air only under otherwise identical circumstances served as control animals.

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

14H-benz[4,5]isoquino[2,1-a]perimidin-14-one

EC Number:

229-904-5

EC Name:

14H-benz[4,5]isoquino[2,1-a]perimidin-14-one

Cas Number:

6829-22-7

Molecular formula:

C22H12N2O

IUPAC Name:

14H-benz[4,5]isoquino[2,1-a]perimidin-14-one

Test material form:

aerosol dispenser: not specified

Remarks:

migrated information: aerosol

Details on test material:

Test item: Macrolex Rot E2G
Chemical name: 14H-Benz[4,5]isoquino[2, 1-a]perimidin-14-one
CAS name: 14H-Benz[4,5]isoquino[2, 1-a]perimidin-14-one
CAS number: 6829-22-7
Empirical formula: C22H12N2O
Molecular mass: 320.3 g/mol
Purity: 98.9%
Stability: Stability certified for the duration of study (see Appendix)
Storage conditions: room temperature
Appearance: solid, red powder
Structural formula:

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose only

Vehicle:

air

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

Actual concentration of 1817 mg/m³

No. of animals per sex per dose:

Three male and three female rats per concentration were simultaneously exposed under nose-only conditions for 4 h.

Control animals:

yes

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

discriminating conc.

Effect level:

1 817 mg/L air (analytical)

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: Mortality did not occur at the technically maximum attainable concentration of 1817 mg/m³.

Mortality:

Mortality did not occur at the technically maximum attainable concentration of 1817 mg/m³.

Clinical signs:

other: Rats exposed to the test item showed clinical signs (labored breathing, irregular breathing, piloerection).

Body weight:

Significant changes in incremental body weight gain were found in females on day 1.

Gross pathology:

Necropsy revealed light colored areas and/or few gray areas in the lungs of male and female rats on day 14.

Any other information on results incl. tables

Mortality did not occur at the technically maximum attainable concentration of 1817 mg/m³. Rats exposed to the test item showed clinical signs (labored breathing, irregular breathing, piloerection). Significant changes in incremental body weight gain were found in females on day 1. Neither reduction in body temperature nor changes during the reflex measurement were observed. Necropsy revealed light colored areas and/or few gray areas in the lungs of male and female rats on day 14.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

No mortality occurs at the technically maximum attainable concentration of 1817 mg/m³.

Executive summary:

A study on the acute inhalation toxicity of Macrolex Rot E2G (CAS no 6829-22-7) on rats has been conducted in accordance with OECD TG#403 (2009). One group of rats was nose-only exposed to the solid aerosol of the test item at the actual concentration of 1817 mg/m³ (maximum attainable concentration). Rats exposed to air only under otherwise identical circumstances served as control animals.

Mortality did not occur at the technically maximum attainable concentration of 1817 mg/m³. Rats exposed to the test item showed clinical signs (labored breathing, irregular breathing, piloerection). Significant changes in incremental body weight gain were found in females on day 1. Neither reduction in body temperature nor changes during the reflex measurement were observed. Necropsy revealed light colored areas and/or few gray areas in the lungs of male and female rats on day 14.

In summary, there is evidence of low acute inhalation toxicity in rats after exposure (4h) of aerosolized Macrolex Rot E2G (CAS no. 6829-22-7). The LC50 is > 1817 mg/m³.