Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study well documented, test procedure in accordance with OECD203 method, meets generally accepted scientific principles, acceptable for assessment.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

1) Holding: in dechlorinated tap water 2) Test: in artificial water 3) test concentration: only 4 concentration tested

GLP compliance:

not specified

Remarks:

Test accordin SOP (standard operating procedure)

Analytical monitoring:

not specified

Details on test solutions:

Reconstituted freshwater according to DIN 38 412, Part 11, October 1982
Preparation from fully demineralized tap water
Conductivity: max 10 mirco MHO

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish (Brachydanio rerio)
- Age: 4.5 months
- Source: West Aquarium Bad Lauterberg, BRD
- Length at study initiation: 26.4 mm
- Weight at study initiation: 0.19 g
- Food type: "Tetra Min" standard feed for aquarium fish
- Amount: ad libitum

ACCLIMATION
- Acclimation period: about 12 days
- Acclimation conditions (same as test or not): the fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Type and amount of food: "Tetra Min" standard feed for aquarium fish
- Feeding frequency: ad libitum

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

2.5 mmol/l

Test temperature:

22-23 °C

pH:

about 8

Dissolved oxygen:

No data

Salinity:

294 mg/l CaCl2 x 2 H2o
123.3 mg/l MgSO4 x 7 H20
63 mg/l NaHCO3
5 mg/l KCl

Nominal and measured concentrations:

56, 100, 178 and 316 ppm

Details on test conditions:

Volume of water: 2.9 liters
No of animals per test concentration: 10
Feeding: none
Aeration: slowly aerated

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

140 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

The LC50 values are calculated wth the Spaerman-Karber method.

Validity criteria fulfilled:

yes

Conclusions:

The results of the test show a LC50 96 h is > 100 mg/l.

Executive summary:

The result of the acute toxicity to fish was performed according to OECD 203.

The results of the test show a LC50 96 h is > 100 mg/l.

Based on the criteria of the Regulation CE 1272/2008, the substance does not needs to be classified.

Description of key information

LC50 (96h) > 100 mg/l 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The test performed on Acid Yellow 194 follows the OECD 203 gave a LC 50 > 100 mg/l.

The tested substance is to be considered as non toxic for acute fish toxicity. Based on the criteria of the Regulation EC n. 1272/2008, the substance does not need to be classified.